The reduction potential of NADH is -320 mV. To which one of the following electron carriers could NADH donate electrons? 1. -120 mV mV b. -400 mV d. +0.50 mV O Choices (a) and (b) O Choices (a), (d) and (e) O Choices (d) and (e) O Choices (a), (b) and (c) O Choices (a) through (e) c. -320 e. +120 mV
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- What is the reaction potential for oxidation of NADH (for completely passing electrons from NADH to O2) with units? 2. What is the reaction potential for oxidation of FADH2 (from FADH2 to O2) with units? 3. Which reaction has a more negative deltaG?Under standard conditions, NADH reoxidation by the electron-transport chain has a free-energy change equal to –220 kJ/mol. With 100% efficiency, how many ATP could be synthesized under standard conditions? What is the "actual" efficiency given these numbers?1 a) What is meant by the ATP currency exchange ratio? Why does the oxidation of mitochondrial FADH2 generate one less ATP than oxidation of mitochondrial NADH? b) If 12 H+ are moved across the inner mitochondrial membrane by NADH oxidation, and each ATP synthesized requires 3 H+ to move through ATP synthase, why are only 3 ATP molecules produced by oxidation of each NADH?
- Calculate the biochemical standard cell potential for the oxidation of NADH by molecular oxygen O2 + 2NADH + 2 H+ → 2H2O + 2NAD+From the complete oxidation of glucose (glucose → 6CO2), how many total NADH electron carriers are produced?Rotenone is an insecticide that is not toxic to plants, but is highly toxic to fish and insects. (it will also kill mammals, but it doesn’t easily get into our cells). When Rotenone is added to cells in vitro, ATP production stops, oxygen is no longer reduced and NADH builds up. Based on these results, explain what is a possible mechanism of action for Rotenone?
- A new ATP-producing protein is discovered that couples ATP production to the oxidation of NADPH by oxidative phosphorylation. Assume that the value of ΔGo for ATP synthesis is 30 kJ•mol−1. If this protein only produces 1 molecule of ATP per reaction that consumes one NADPH: a. How much free energy is wasted, under standard conditions?b. How many more ATP molecules could be created by a perfectly efficient electron transport chain from one NADPH?It is estimated that each electron pair donated by NADH leads to the synthesis of approximately three ATP molecules, whereas each electron pair donated by FADH2 leads to the synthesis of approximately two ATP molecules. What is the underlying reason for the difference in yield for electrons donated by FADH2 versus NADH?Given what you know about the involvement of nicotinamide nucleotides inoxidative and reductive metabolic reactions, predict whether the followingintracellular concentration ratios should be 1, > 1, or < 1. Explain youranswers.(a) [NAD+] >[NADH](b) [NADP+] >[NADPH](c) Since NAD+ and NADP+ are essentially equivalent in their tendency to attract electrons, discuss how the two concentration ratios might bemaintained inside cells at greatly differing values.
- A substantial amount of energy is put into the photosynthetic electron transport to extract electronsf from water and to elevate the energy level of ferredoxin to a very strong reductant. The amount of energy in one NADPH is 218 kJ.mol-1. What is the percentage of this energy conserved in NADPH for the production of one mole of oxygen? What happened to the rest of the energy?What is the P:O ratio for electrons entering the electron-transport chain that originate from reoxidation of acyl-CoA dehydrogenase? A. greater than the P:O ratio for NADH reoxidation B. approximately the same as the P:O ratio for NADH reoxidation C. less than the P:O ratio for NADH reoxidation but more than the P:O ratio for Complex II reoxidation D. approximately the same as the P:O ratio for Complex II reoxidation E. less than the P:O ratio for Complex II reoxidationThe values of the reduction potential (Eº') of the conjugated redox pairs NAD+/NADH and pyruvate / lactate are -0'32 V and -0'19 V respectively. a) Which of the two conjugated pairs has a greater tendency to lose electrons? Reason for it. b) What is the strongest oxidizing agent? Reason for it. c) If we start with 1M concentrations of each reagent and product at pH 7.0 in which sense the reaction will pass pyruvate + NADH + H+ ⇄ lactate + NAD+ d) What is the standard free energy variation (ΔGº’ at 25ºC) for the conversion of pyruvate to lactate? e) What is the equilibrium constant of this reaction?