Enzyme kinteics of amylase using l2 in Kl was used to determine starch conc at t = 0 min andt = 1 min. Tubes were set absorbance measured after 1 minute of incubation. The reactionwas halted with HCl and 5 mL of I, in KI indicator was added. 1 ml of each was used assample in the spectrophotometer.Using this table,Concentration ofVolume ofVolume ofVolume of l2Test tube stock starch,starchdistilledin KI solution, Absorbancemg/mLsolution, mL water, mLmL1100.00092100.44.650.33063100.84.20.589110|1.23.80.840910|1.63.41.0885102.03.01.2572What is the standard curve for the conc. of the starch?

The standard curve allows estimating the concentration of unknown samples by comparing them to known…

Answered: Enzyme kinteics of amylase using l2 in…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…
A paste of starch was tested using Benedict’s test, the Iodine Test, and Fehling’s Test. Before hydrolysis, Benedict’s test and Fehling's test results were both negative while Iodine’s test is positive. After adding 0.5 mL of conc. HCl to the paste, another 5 drops of NaOH were added and was tested using Fehling’s, Benedict’s Test, and Iodine Tests. What would be the theoretical result of the three different tests? Explain the chemical reaction that happened and the principle/mechanism behind the reactions.
How do I calculate the final concentration? I just need one solution to walk through the steps. Prepare eight standards of glucose with your 1 mM glucose solution, each with a final volume of 10 mL. Complete the table on the next page, indicating what your final concentration of glucose will be for each standard, in mM. Volume of Potassium Ferrocyanide Solution Volume of Enzyme Solution Volume of 1 mM glucose solution Final volume of solution Final Concentration of glucose in final solution (mM) 2.50 mL 1.00 mL 0.00 mL 10.00 mL 2.50 mL 1.00 mL 0.50 mL 10.00 mL 2.50 mL 1.00 mL 1.00 mL 10.00 mL 2.50 mL 1.00 mL 2.00 mL 10.00 mL 2.50 mL 1.00 mL 3.00 mL 10.00 mL 2.50 mL 1.00 mL 4.00 mL 10.00 mL 2.50 mL 1.00 mL 5.00 mL 10.00 mL 2.50 mL 1.00 mL 6.00 mL 10.00 mL
For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.
I need help with my homework this question is very confusing How long would it take for a solution made by diluting 2.5 ml of #1 with 7.5 ml of H2O to react with 10 ml of #2?
points) You have to make 50mL of 0.05M HCI from a 10M HCI stock solution, but the onlymeasuring devices available are 100mL graduated cylinders and 10mL serological pipes. Howcould you accurately make the dilute HCI solution (clearly explain)?
Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.
The absorbance values for different BSA standards. How can the protein concentration be calculated? Which absorbance value should be used?
Chlorophyll is the green pigment in the leaves of the plant, carotene imparts orange coloration while anthocyanin is known as the purple pigment. In one of their laboratory, a group was tasked to confirm the presence of these pigments on a certain leaf. After crushing several leaves, they soak it in isopropyl paper strip was dried and the group got the following measurements from the submerged tip of the paper strip: solvent font - 9.9cm, purple ink -8.6cm, yellowish orange ink - 6.4cm and green ink - 4.2 retention factor of each pigment. (d) Rank each pigment according to their solubility in the given solvent.
A certain amount of white solid was dissolved in enough water and gives a colorless solution. A portion of the solution was added with 15% solution of alpha-naphthol solution and a few drops of conc. H2SO4 forming a violet-colored ring. Another portion was added with drops of Seliwanoff’s reagent and showed no change after being warmed in a bath. Another portion was added with equal amounts of Fehling’s A and B reagent giving a red precipitate in a blue solution after warming in a bath. a) Cite an experimental result confirming that the solid is a carbohydrate b) Is the solid starch, fructose or glucose? Support your answer.
Phenol Red Test Question: Assuming you tested an organism using Phenol Red glucose and Phenol Red Base broths, which of the combinations of results in the following table would be reliable. Interpret each of the combinations and explain how the results might be obtained
Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…

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