based on the chart answer the following:1. which proteins will be most excluding use gel filtration chromotagrpahy 2. which proteins will migrate closer to the anode using SDS- PAGE3. which protein will have the most net positive charge at a ph of 7.4 **Protein Properties Overview**This table lists a series of proteins along with their respective molecular weights and isoelectric points (pI). The molecular weight is expressed in Daltons (Da).| Protein | Molecular Weight (Da) | pI ||---------------------------|-----------------------|-----|| Aldolase | 40,000 | 10.1|| Cytochrome C | 14,000 | 9.2 || Lactate dehydrogenase | 18,000 | 8.5 || Ornithine decarboxylase | 75,000 | 4.1 || PEP carboxylase | 74,000 | 5.0 || Pyruvate kinase | 62,000 | 6.0 |**Explanation:**- **Molecular Weight (Da):** This indicates the mass of the molecule. Larger values signify heavier proteins. - **pI (Isoelectric Point):** This is the pH at which the protein carries no net charge. Different proteins have unique pI values, influencing their behavior in various pH environments.This data is crucial for understanding the biochemical properties and behaviors of these proteins, useful in fields such as biochemistry and molecular biology.

Chromatographic techniques:These are a diverse group of laboratory methods that are used for the…

Answered: gel filtration chromotagrpahy

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

Related questions

Question

based on the chart answer the following:

1. which proteins will be most excluding use gel filtration chromotagrpahy

2. which proteins will migrate closer to the anode using SDS- PAGE

3. which protein will have the most net positive charge at a ph of 7.4

Expert Solution

Step 1: Explain Chromatography, Electrophoresis and pH

Chromatographic techniques:

These are a diverse group of laboratory methods that are used for the separation and analysis of mixtures of substances.
These are widely employed in various scientific disciplines such as chemistry, biochemistry, pharmacology and environmental science.
Thes techniques relies on the differential distribution of components within a sample between a stationary phase and a mobile phase which allows the separation and identification of individual compounds.
There are different types of chromatographic techniques which include GC, HPLC, LLC, Ion exchange chromatography, SEC, TLC, Paper chromatography, Affinity chromatography, HIC, Chiral chromatography, SPE, etc.,
The specific chromatographic technique is selected depending on the nature of the sample and the properties of the compounds to be separated.

Electrophoretic techniques:

These are a group of laboratory methods used to separate and analyze charged molecules i.e., DNA, RNA, proteins, and particles, based on their mobility in an electric field.
These techniques are essential tools in various scientific disciplines such as molecular biology, biochemistry, genetics and materials science.
There are different types of electrophoretic techniques which include Gel electrophoresis, PAGE, CE, Proteomics electrophoresis, Agarose Gel electrophoresis, PFGE, IEF, EMSA, Particle electrophoresis, Electrophoresis in Microfluidic Devices, etc.,

pH:

It is a measure of the acidity or alkalinity) of a solution.
It quantifies the concentration of H⁺ ions in a solution.
The scale typically ranges from 0 to 14, by considering 7 as neutral.
When pH of a solution is below 7, it is considered as acidic solution.
When pH of a solution is above 7, it is considered as basic or alkaline solution.
It is defined as the negative logarithm (base 10) of the hydrogen ion concentration i.e., pH = -log[H⁺].