What will be the color of saliva extraction with iodine in 3, 6 9, 12, 15, and 18 mins if: you put 5 ml of starch soln. and you heat it to boiling after that you will add 1 ml of saliva. Cool and then continue to heating after that you will place it in a water bath at 60 degree Celsius. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results.

Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.192   and A650=0.090, taking into account the dilution factor (100), and the total volume of extract (45). Note your answer to 2 decimal places.

A paste of starch was tested using Benedict’s test, the Iodine Test, and Fehling’s Test. Before hydrolysis, Benedict’s test and Fehling's test results were both negative while Iodine’s test is positive. After adding 0.5 mL of conc. HCl to the paste, another 5 drops of NaOH were added and was tested using Fehling’s, Benedict’s Test, and Iodine Tests. What would be the theoretical result of the three different tests? Explain the chemical reaction that happened and the principle/mechanism behind the reactions.

Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.

The absorbance values for different BSA standards.  How can the protein concentration be calculated?  Which absorbance value should be used?

What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45

Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…

how to make 100ml of TBST buffer from 1X TBS (tris buffered saline solution) and 0.1% tween-20. I am just confused how to do the calculations when it is 1X TBS and not 10X TBS. Please show calculations

Blood added with 25 ml distilled water + submerged in water bath, added 1-2 drops of 10% acetic acid = brownish precipitate the brown precipitate was then filtered. The filtrate is placed on the test tube for about 2ml and was added with Benedict's reagent (4 drops). Then was boiled in the water bath. The solution became green.What is the chemical equation?

To identify a positive control in your lab report, should you list any test tube with positive results? Why or why not? What is a negative control? Which substance is a negative control for each of the tests in this lab exercise?