What is the relationship of concentration and absorbance based on the table and what are the changes in the absorbance value mean?
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What is the relationship of concentration and absorbance based on the table and what are the changes in the absorbance value mean?
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- BSA Volume (1.0mg/ml) (ul) [BSA] (ug/ml) Absorbance 595nm Net Abs 595nm 0.0 0.371 10.0 0.559 20.0 .707 25.0 .902 Please help me calculate BSA and Net Abs (explaination and details pls)K 1 1 H202 (mL) Rate (abs/secs at 500 nm) Absorbance tubes 2 &3 Absorbance tubes 4 &:Absorbance tubes 6 & Absorbance 8 & 9 Time 2. 2.5 0.001907143 0.065 0.044 0.007 0.059 0.041 3 1.75 0.001721429 20 0.017 0.027 0.008 4 1.25 0.001728571 40 0.074 0.063 0.044 0.037 5 0.75 0.001548214 60 0.117 0.103 0.085 0.079 6. 80 0.163 0.148 0.13 0.117 7. 100 0.211 0.195 0.178 0.16 8 120 Figure 1: 10 0.0025 11 12 13 etoxidaze rate 14 0.002 15 16 17 0.0015 18 19 20 21 0.001 22 23 24 25 0.0005 26 27 28 29 30 0.5 1.5 2.5 31 H202 Concentration (ml) 32 Sheet1 (wu 0os ze sas/sqe) azeCalculate the Vmax with the parameters given. All parameters may not be needed to calculate. Kcat = 130 s^-1 Vo = 3.0 μMs^-1 S = 10 μM Et = 0.15 µM
- Construct a standard curve (absorbance versus protein concentration in the standards) Conc. (mg/ml) 0 0.25 0.5 0.75 1.0 Absorbance 0.000 0.126 0.249 0.361 0.464Siturul alORK ING )A mother brings her 251b child Into the ER with q very high fevero she has already ad ministered l2 4sp of Tylenol at home. She has brought the bottle with her and the label states: Acetamaino phere syrmp 325 mg / 5mLo Administer 10-15 mg /89 every 4 hrs as needed . How many milligrams oL Tylenot has she givern? Was this asafe dose to give the child?Rx Ephedrine sulfate (fz. pt = -0.13°C). 2%Sodium chloride MW 58.5Purified water qs ad. 30 mL How much sodium chloride should be used to make this eye solution isotonic with tears? the answer is 224mg
- CAR) All una Red stock soluton concentration: wis strue Somple #yelume dilution 1 ete total tock AR volume 50.00m 50. 00mL 50.00L AR COC conentrastion Absorbance Q dilutibn 2 dilution 3 10 O. 16.4 20 0. 237 turn dilution 4 25 So00 O. 392 shift unknarn 1 0.485 (Include a printed copy of the colibratlon curve) Colibrotion urve cayotion: R2 valveYou obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) Answer Skip ExitWhat is measured at the A280 absorbance values?
- A 24-year-old female has just delivered a baby and will receive 1.9 grams/hr of magnesium sulfate for eclampsia. How many mEq of magnesium sulfate (MW 120; valence 2) will the patient receive in 24 hours?The following is HPLC data. Using the standard curve determine the concentration of caffeine in each sample in µg/µl: Sample Peak area Volume of sample Amount of caffeine µg/ µl 1 1305490 1 µl 2 7836463.3 1 µl 3 3476413.7 1 µl 4 2936950.7 1 µl 5 15452894 1 µl 6 958353.7 1 µl 7 592743.3 1 µl 8 557099 1 µl 9 653410.7 1 µl 10 734443.7 1 µlPROBLEM 12.27 (a) Use Table 12.5 to calculate the osmotic pressure of the hemodi- alysis solution at 25 °C. (b) If the osmotic pressure of blood at 25 °C is 7.70 atm, what is the direction of solvent movement across the semipermeable mem brane in dialysis? (Blood to dialysis solution or dialysis solution to blood)
