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what are the principle chemical reaction involved in each test?

what are the principle chemical reaction involved in each test?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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what are the principle chemical reaction involved in each test?
Apparatus and Reagents Test tubes, 13 x 100 Droppers Lucas reagent (dissolve 27 g anhydrous ZnCl2 in 18 Lucas Test Place 10 drops test compounds in separate test tubes. Add 10 drops of Lucas reagent. Shake vigorously and note the appearance of cloudiness or insoluble layer. ml conc. HCI) Tollens' Test Apparatus and Reagents Test tubes, 13 x 100 Droppers Tollens reagent (Solution A: dissolve 0.25g AgNO3 in 4.3 mL H20; Solution B: dissolve 0.3g NaOH in 4.2 ml H20; Add conc. NH3 to solution A dropwise until a brown precipitate is formed. Add solution B dropwise until the solution is clear) Boiling Water bath Place 20 drops of freshly prepared Tollens reagent in separate test tubes. Add 10 drops test compounds to each test tube. Mix well and place in boiling water bath for 5 minutes. Take note of any change. Ceric Nitrate Test Place 10 drops test compounds in separate test tubes. Add 10 drops of Ceric ammonium nitrate reagent. Note the change in color. Test tubes, 13 x 100 Droppers Ceric ammonium nitrate reagent (Dissolve 45g of Ce(NHa)2(NO3)6 in 115 mL 2M HNO3 Tabulation of Result Fill up the table below for the expected results for the following tests and sample compounds. Tollens Dichromate Test Hydroxamic acid Test DNPH lodoform Lucas Ceric nitrate (Jones Test) Test Test Test Test Test 1° ROH (Methanol) 2° ROH (2-Propanol) 3° ROH (tert-Butanol) Aldehyde (Formaldehyde) Ketone (Acetone) Ester (Ethyl acetate) Post Laboratory - Group Discussion Questions Draw the structures of methanol, 2-propanol, tert-butanol, acetone, acetaldehyde and ethyl acetate. Indicate the differences in their structures. Write the equation of the reactions of each compound with the reagents abov Write NR if there is no reaction. What are the principles / chemical reaction involved in each chemical test? Indicate the functional groups that correspond to the IR signals.
Transcribed Image Text:Apparatus and Reagents Test tubes, 13 x 100 Droppers Lucas reagent (dissolve 27 g anhydrous ZnCl2 in 18 Lucas Test Place 10 drops test compounds in separate test tubes. Add 10 drops of Lucas reagent. Shake vigorously and note the appearance of cloudiness or insoluble layer. ml conc. HCI) Tollens' Test Apparatus and Reagents Test tubes, 13 x 100 Droppers Tollens reagent (Solution A: dissolve 0.25g AgNO3 in 4.3 mL H20; Solution B: dissolve 0.3g NaOH in 4.2 ml H20; Add conc. NH3 to solution A dropwise until a brown precipitate is formed. Add solution B dropwise until the solution is clear) Boiling Water bath Place 20 drops of freshly prepared Tollens reagent in separate test tubes. Add 10 drops test compounds to each test tube. Mix well and place in boiling water bath for 5 minutes. Take note of any change. Ceric Nitrate Test Place 10 drops test compounds in separate test tubes. Add 10 drops of Ceric ammonium nitrate reagent. Note the change in color. Test tubes, 13 x 100 Droppers Ceric ammonium nitrate reagent (Dissolve 45g of Ce(NHa)2(NO3)6 in 115 mL 2M HNO3 Tabulation of Result Fill up the table below for the expected results for the following tests and sample compounds. Tollens Dichromate Test Hydroxamic acid Test DNPH lodoform Lucas Ceric nitrate (Jones Test) Test Test Test Test Test 1° ROH (Methanol) 2° ROH (2-Propanol) 3° ROH (tert-Butanol) Aldehyde (Formaldehyde) Ketone (Acetone) Ester (Ethyl acetate) Post Laboratory - Group Discussion Questions Draw the structures of methanol, 2-propanol, tert-butanol, acetone, acetaldehyde and ethyl acetate. Indicate the differences in their structures. Write the equation of the reactions of each compound with the reagents abov Write NR if there is no reaction. What are the principles / chemical reaction involved in each chemical test? Indicate the functional groups that correspond to the IR signals.
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