You are given as following : 20 µl pure LDH on ice, 2.0ml of 6mM NAD+, 2.0ml of 150mM lactate, and 0.14M CAPS buffer. LDH reaction cocktail has final concentration of 1mM NAD+ and 25mM lactate in 0.14M CAPS buffer. LDH activityis measured by mixing 10 µl of LDH sample and 990 µl LDH reaction cocktail before getting ∆A340/min reading on spectrometer. (a). Describe in detail how you would prepare for your LDH reaction cocktail including how to make dilutions.
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You are given as following : 20 µl pure LDH on ice, 2.0ml of 6mM NAD+, 2.0ml of 150mM lactate, and 0.14M CAPS buffer.
LDH reaction cocktail has final concentration of 1mM NAD+ and 25mM lactate in 0.14M CAPS buffer. LDH activityis measured by mixing 10 µl of LDH sample and 990 µl LDH reaction cocktail before getting ∆A340/min reading on spectrometer.
(a). Describe in detail how you would prepare for your LDH reaction cocktail including how to make dilutions.
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- Data for standard glucose concentrations Glucose concentration (mm) 0 0.5 1 1.5 -~~ 2 2.5 A Data for plasma samples B Patient Timepoint (hours) C D E 0 0.5 1 2 0 0.5 1 2 0 0.5 1 2 1. Draw a standard curve of absorbance at 420nm against glucose concentration in mmoles/L. We recommend you use Excel or a similar programme to insert the graph Alternatively, you can draw by hand on graph paper and insert a photo of the graph. OON 0 0.5 1 Absorbance 1 0.000 0.477 0.947 1.234 1.733 2.160 2 0 0.5 1 Absorbance Absorbance 2 0.000 0.482 0.941 1.236 1.721 2.276 Absorbance Glucose concentration Glucose concentration Abs 1 Abs 2 Average from graph (mM) in plasma (mm) 0.635 0.632 0.629 1.368 1.370 1.369 1.382 1.388 1.385 1.151 1.147 1.149 0.566 0.844 0.869 0.772 0.395 0.397 0.399 0.850 0.858 0.854 0.738 0.744 0.741 0.539 0.531 0.535 0.776 0.780 0.778 1.475 1.481 1.478 1.446 1.444 1.445 1.302 1.296 1.299 0.560 0.563 0.848 0.846 0.871 0.870 0.774 0.773 Average 0.000 0.480 0.944 1.235 1.727 2.218 0.440…Abs undiluted 0.208 Diluted (1/10) 0.105 Diluted (1/20) 0.09 Use the chart above to back calculate the protein concentration of the LDH sample based on the Abs562 measurements of the undiluted and 2 diluted LDH samples. Show calculation for each.Given the following data set, which cuvettes could be used to make a standard curve for absorption data, including a zero point? Check all that apply. Cuvette 1: 0.5 ml water Cuvette 2: 0.5 ml 2 mg/dl protein solution Cuvette 3: 0.5 ml 4 mg/dl protein solution Cuvette 4: 0.5 ml 6 mg/dl protein solution Cuvette 5: 0.5 ml 8 mg/dl protein solution Cuvette 6: 0.5 ml Patient 1 sample Cuvette 7: 0.5 ml Patient 2 sample Cuvette 8: 0.5 ml Patient 3 sample Cuvette 9: 0.5 ml Patient 4 sample O Cuvette 1 O Cuvette 9 Cuvette 7 OCuvette 5 O Cuvette 3 OCuvette 8 O Cuvette 2 OCuvette 4 O Cuvette 6.
- Recommend an appropriate dosage of digoxin for this patient given a trough digoxin concentration of 2.7 ng/mL.Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn and coconut water 10%. Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn 5gm/100ml. If you need anything else then you add accordingly. BAP has a MW of 225.3. Sucrose and Myo-inositol is not mentioned in the question. These you need to add. Consistency of the medium is also mentioned.What is the experimentally-determined minimum inhibitory concentration of the antibiotic being tested in this picture, based on the results shown in the picture? 0.06 0.125 0.25 1.0 μg/ml 8.0 µg/ml 0.5 µg/ml ○ 0.06 µg/ml µg/ml 0.5 1.0 2.0 4.0 8.0
- A 50-year-old diabetic comes to the laboratory to have rou- tine bloodwork drawn. His physician orders basic metabolic panel and a lipid profile. After the blood clots, the techni- cian spins down the specimen to find that the serum looks like milk. The test results are as follows: Na: 140 mEq/L K: 4.0 mEq/L Cl: 101 mEq/L CO2: 28 mg/dL Glucose: 455 mg/dL BUN: 24 mg/dL Creatinine: 1.1 mg/dL Phosphate: 3.7 mg/dL Calcium: 8.8 mg/dL ALP: 250 IU/L AST: 35 IU/L ALT: 29 IU/L Cholesterol: 500 mg/dL Triglycerides: 1200 mg/dL HDL: 35 mg/dL LDL: 160 mg/dL What causes the diabetic's serum to look like milk? What lipid test results do you expect to be elevated? How would this specimen fit into Fredrickson's classification scheme?You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.570 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.262 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
- A%252F%252Fbbniagaraccc.sln.suny.edu%252Fwe ignment Saved The drip rate of a dobutamine IV depends on the dose (in mcg/min), the weight of the patient, and the dobutamine solution concentration (in mg/ml). For a concentration of 2 mg/ml, the drip rate (in ml/hour), is described by the following formula: Drip rate = 0.03 [patient body mass (kg) × dose infusion rate (mcg/kg-min)] For a patient weighing 209 pounds and a dose infusion rate of 20 mcg/min for every kilogram of body mass, what is the IV drip rate? ml/hrWhat rate in mL/hr of Isosource VHN would provide 1.6 g protein/kg/day if run for 24 hours for a patient weighing 68 kg? Round to the nearest whole number, do not enter units EN Product Кcal Protein /mL g/L General Formulations Jevity 1.5 1.5 63.4 Isosource VHN 1 62 TwoCal HN 2 83 Renal Formulations Nepro 1.8 81 Renalcal 2 34.4 Supplena 1.8 45 Glucose Control Formulations Glucerna 1 41 Choice DM 1 45Absorbance of urea for a diabetic patient is 0.335 while 0.214 is the absorbance of standard kit for the same test, calculate the concentration of this test ? Concentration of standard kit = 50 mg/dl. Repl...