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Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.

Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.

In this kinetic experiment, the focus is on evaluating an enzyme's ability to hydrolyze the β-lactam antibiotic, amoxicillin. The process is monitored by measuring changes in absorbance at 240 nm. The experiment is conducted at 30°C using a buffer solution with a concentration of 100 μM sodium phosphate at pH 7.0. Initially, a 50 μM solution of amoxicillin is used, and absorbance is recorded every 10 seconds over a 2-minute period. ### Collected Data The absorbance readings at different time intervals are presented below: | Time (s) | Absorbance (mΔAbs) | Time (s) | Absorbance (mΔAbs) | |----------|--------------------|----------|--------------------| | 0 | 542.5 | 70 | 323.9 | | 10 | 508.5 | 80 | 300.5 | | 20 | 474.4 | 90 | 278.8 | | 30 | 440.4 | 100 | 258.6 | | 40 | 406.4 | 110 | 239.9 | | 50 | 376.3 | 120 | 222.6 | | 60 | 349.1 | | | ### Instructions for Analysis - Plot a graph of absorbance versus time using the given data. - Identify the initial rate of reaction by drawing a line from the plotted data that best represents the data points in their initial linear phase. This experiment demonstrates how enzymatic activity can be quantitatively analyzed through absorbance changes, illustrating the enzyme kinetics involved in hydrolyzing amoxicillin.
Transcribed Image Text:In this kinetic experiment, the focus is on evaluating an enzyme's ability to hydrolyze the β-lactam antibiotic, amoxicillin. The process is monitored by measuring changes in absorbance at 240 nm. The experiment is conducted at 30°C using a buffer solution with a concentration of 100 μM sodium phosphate at pH 7.0. Initially, a 50 μM solution of amoxicillin is used, and absorbance is recorded every 10 seconds over a 2-minute period. ### Collected Data The absorbance readings at different time intervals are presented below: | Time (s) | Absorbance (mΔAbs) | Time (s) | Absorbance (mΔAbs) | |----------|--------------------|----------|--------------------| | 0 | 542.5 | 70 | 323.9 | | 10 | 508.5 | 80 | 300.5 | | 20 | 474.4 | 90 | 278.8 | | 30 | 440.4 | 100 | 258.6 | | 40 | 406.4 | 110 | 239.9 | | 50 | 376.3 | 120 | 222.6 | | 60 | 349.1 | | | ### Instructions for Analysis - Plot a graph of absorbance versus time using the given data. - Identify the initial rate of reaction by drawing a line from the plotted data that best represents the data points in their initial linear phase. This experiment demonstrates how enzymatic activity can be quantitatively analyzed through absorbance changes, illustrating the enzyme kinetics involved in hydrolyzing amoxicillin.
The image displays a graph of absorbance versus time, likely from a spectrophotometry experiment. **Graph Details:** - **Title:** The graph doesn’t have a specific title but represents absorbance data over time. - **Y-Axis (Vertical):** The y-axis is labeled as "Absorbance (mAbs)" with values ranging from 225 to 550 milli-absorbance units. - **X-Axis (Horizontal):** The x-axis is labeled as "time(s)" with values ranging from 0 to 120 seconds. **Graph Description:** - A red dashed line slopes downward, indicating a decrease in absorbance over time. - It starts near the top-left corner, around 550 mAbs at time zero and slopes down to approximately 225 mAbs at around 90 seconds. - Two orange points are marked on the line at approximately 10 seconds (540 mAbs) and 70 seconds (400 mAbs), possibly indicating specific data points or measurements. This type of graph can be used to analyze the rate of reaction in a chemical experiment, demonstrating how the concentration of a substance changes over time.
Transcribed Image Text:The image displays a graph of absorbance versus time, likely from a spectrophotometry experiment. **Graph Details:** - **Title:** The graph doesn’t have a specific title but represents absorbance data over time. - **Y-Axis (Vertical):** The y-axis is labeled as "Absorbance (mAbs)" with values ranging from 225 to 550 milli-absorbance units. - **X-Axis (Horizontal):** The x-axis is labeled as "time(s)" with values ranging from 0 to 120 seconds. **Graph Description:** - A red dashed line slopes downward, indicating a decrease in absorbance over time. - It starts near the top-left corner, around 550 mAbs at time zero and slopes down to approximately 225 mAbs at around 90 seconds. - Two orange points are marked on the line at approximately 10 seconds (540 mAbs) and 70 seconds (400 mAbs), possibly indicating specific data points or measurements. This type of graph can be used to analyze the rate of reaction in a chemical experiment, demonstrating how the concentration of a substance changes over time.
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