Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.

A new oxygen transport protein that exhibits cooperative binding has been isolated and is beingstudied in the lab. Calculate the KD value if Y = 0.76 when pO2 = 18 torr (assume n = 2.5). Howdoes this compare to the KD value for hemoglobin? Does this protein bind more or less tightly tooxygen compared to hemoglobin?

Consider the following set of data and answer the fo

An experiment was carried out to measure the reaction rate of hydrolysis of acetylcholme (substrate) with serum enzymes (Eadie, 1949). In the experiment, two experiments were conducted, namely experiment 1 without using a prostigmine inhibitor and experiment 2 using a prostigmine inhibitor at 1.5 x 10^-7 mol/l. the data obtained are:     a. Is prostigmine competitive or noncompetitive inhibitor? b. determine the value of km and rmax for the two experiments, compare

Penicillin is hydrolyzed and thereby rendered inactive by penicillinase, an enzyme present in some resistant bacteria.  The mass of this enzyme in Staphylococcus aureus is 29.6 kd.  The amount of penicillin hydrolyzed in 1 minute in 10 ml solution containing 10–9 g of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change during the assay. [Penicillin] µM            Amount hydrolyzed (nanomoles) 1                                  0.11 3                                  0.25 5                                  0.34 10                                0.45 30                                0.58 50                                0.61   a) Plot V0 versus [S] and 1/V0 1/[S]. Does penicillinase appear to obey Michaelis-Menten kinetics?   b) What is the value of KM?   c) What is the value of Vmax?   d) What is the turnover number of penicillinase under these conditions?

WAs there a clear lactate threshold point?

The air supply to a fermenter was turned off for a short period of time and then restarted. A value for C* of 7.3 mg/l of has been determined for the operating conditions. Use the tabulated measurements of dissolved oxygen (DO) values in the following table to estimate the oxygen uptake rate and kla in this system. Time (min) DO (mg/l) -1 3.3 Air off 3.3 1 2.4 2 1.3 3 0.3 4 0.1 0.0 Air on 0.0 7 0.3 1.0 9. 1.6 10 2.0 11 2.4 12 2.7 13 2.9 14 3.0 15 3.1 16 3.2 17 3.2

The graph is of initial velocity versus substrate concentration. Estimate Vmax from the direct graph of v versus [S] using the plot. Estimate KM from the direct graph of v versus [S] using the plot, also.

When determining CFUs for a dilution of the same sample at 10-2 and 10-3 which of the following results would seem the most accurate to you? Explain why in the space below. A. 110,000 CFU/mL for 10-2; and 140,000 CFU/mL 10-3 B. 110,000 CFU/mL for 10-2; and 14,000 CFU/mL 10-3 C. 10,000 CFU/mL for 10-2; and 140,000 CFU/mL 10-3 D. none of the above

Calculate the patient’s INTAKE during your 12-hour shift (7am - 7pm): (Show your computation and conversion process)   08:00am: Two pieces of toast, 2 cups of oatmeal, 12 oz orange juice 10:00am: Two 8 oz of coffee 11:00am: 24 oz of ice chips 12:00pm: IV infusion of Zosyn 50 mL, 2 mL IV push Zofran and 10 cc saline IV flush 12:30pm: house salad, 12 oz soda, three 12 oz popsicles 2:00pm: One pack of red blood cells (250mL) 3:00pm: 2 mL Morphine and 10 cc saline flush IV 5:15pm: 10 cc saline flush IV 4:00pm-7:00pm: Normal Saline IV 150 cc/hr