How do I calculate the final concentration? I just need one solution to walk through the steps.    Prepare eight standards of glucose with your 1 mM glucose solution, each with a final volume of 10 mL. Complete the table on the next page, indicating what your final concentration of glucose will be for each standard, in mM. Volume of Potassium Ferrocyanide Solution Volume of Enzyme Solution Volume of 1 mM glucose solution Final volume of solution Final Concentration of glucose in final solution (mM) 2.50 mL 1.00 mL

If a protein concentration is 1 mg/ml and diluted it by 1/10;what is the final concentration?

Match the common laboratory scenarios to the correct buffer required using the guide below. Molecule pKa Value(s) Acetic acid 4.8 Carbonic acid 6.1, 10.2 Citric acid 3.1, 4.8, 5.4 HEPES 7.5 Phosphoric acid 2.1, 7.1, 12.3 TRIS 8.3                                                                                   Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments.                                                                                   Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer.                                                                                   Dissolution of lysozyme requires pH 3 buffer to maintain activity.                                                                                   Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer.…

Is there a difference between pH measurements using the pH meter and the pH paper? Compare the initial pH and the final pH of each sample. What was the most effective buffer? What was the least effective?  Solución pH inicial pH final Diferencia water 7.45 2.48   0.1 M NaCl 7.28 2.03   milk 6.78 6.45   buffer de fosfato 8.76 7.78     **** Calculate the difference (subtract) the initial pH - final pH values ​​to identify the effectiveness of each solution as a buffer. Values ​​with little difference mean that a less drastic change occurred and are therefore more effective. On the contrary, values ​​by much difference reflect drastic changes in pH and are less effective. What do buffers do and why are they important in biological systems?