Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 8, Problem 8.11PS
Inverted Vesicles. An important advance in transport research was the development of methods for making closed membrane vesicles that retain the activity of certain transport systems. One such system uses resealed vesicles from red blood cell membranes, in which the orientation of membrane proteins may be either the same as in the intact cell (right side out) or inverted (inside out). Such vesicles have demonstrable ATP-driven Na+/K+ pump activity. By resealing the vesicles in one medium and then placing them in another, it is possible to have ATP, sodium ions, and potassium ions present inside the vesicle, present outside the vesicle, or not present at all.
- (a) Suggest one or two advantages that such vesicles might have compared to intact red blood cells for studying the Na+/K+ pump. Can you think of any possible disadvantages?
- (b) For the inverted vesicles, indicate whether each of the following should be present inside the vesicle, outside the vesicle, or not present at all in order to demonstrate ATP hydrolysis: Na+, K+, and ATP.
- (c) If you were to plot the rate of ATP hydrolysis as a function of time after initiating transport in such inverted vesicles, what sort of a curve would you expect to obtain?
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No free lunch. Explain why maintaining a high concentration of CO2
CO2 in the bundle-sheath cells of C4C4 plants is an example of active
transport. How much ATP is required per CO2CO2 to maintain a high
concentration of CO2CO2 in the bundle-sheath cells of C4C4 plants?
Transport. Provided below is an abstracted equation describing an active cell membrane
transport from outside the cell to the cytoplasm, involving a membrane carrier (Cmemb), a
substrate (Sin) (growth factor etc.) to be carried and released (Sout) into the cytoplasm.
a) What kind of feedback mechanism is restricting this process?
b) Construct a Forrester Diagram
Membrane Transport Carrier, C:C, +S.
memb
k₁
k_₁
C memb
memb
+ S
out
Solid Lines. There are two applications in the construction of Forrester diagrams where
solid lines can be used to connect state variables. Please name them:
a)
b)
Multiple Choice. Imagine a solution containing only water, sodium, and glucose. If a voltage is applied to the solution, glucose would move:
(a) There would be no net movement of glucose
(b) In the opposite direction of sodium
(c) In the same direction and speed as sodium
(d) In the same direction as sodium, but slower.
(e) In the same direction as sodium, but faster.
Chapter 8 Solutions
Becker's World of the Cell (9th Edition)
Ch. 8 - What is the difference between the concentration...Ch. 8 - Prob. 8.2CCCh. 8 - A researcher is studying a fern that is shown to...Ch. 8 - How are carrier proteins and channel proteins...Ch. 8 - How would you determine whether a specific...Ch. 8 - Both the Na+/glucose symporter and the Na+/K+ pump...Ch. 8 - You are studying the energetics of transport of...Ch. 8 - True or False? Indicate whether each of the...Ch. 8 - Telling Them Apart. From the following list of...Ch. 8 - Mechanisms of Transport. For each of the following...
Ch. 8 - Prob. 8.4PSCh. 8 - Prob. 8.5PSCh. 8 - Prob. 8.6PSCh. 8 - QUANTITATIVE Sodium Ion Transport. A marine...Ch. 8 - Prob. 8.8PSCh. 8 - Prob. 8.9PSCh. 8 - QUANTITATIVE The Calcium Pump of the Sarcoplasmic...Ch. 8 - Inverted Vesicles. An important advance in...Ch. 8 - Ouabain Inhibition. Ouabain is a specific...
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