Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 8, Problem 4P
The diagram below shows a DNA duplex. The template strand is identified, as is the location of the
Assume this region contains a gene transcribed in a bacterium. Identify the location of promoter consensus sequences and of the transcription termination sequence.
Assume this region contains a gene transcribed to form mRNA in a eukaryote. Identify the location of the most common promoter consensus sequences.
If this region is a eukaryotic gene transcribed by RNA polymerase III, where are the promoter consensus sequences located?
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What is the point of DNA replication? ____________________________
When & where does replication occur? _____________________________
What is the point of transcription? _______________________________ Where does it occur? _____________________________
What are three nucleotides together called on mRNA? (ie: ACA)__________
The mRNA codons can be used in a chart to find: ____________________
What molecule contains an anti-codon? ______________________ During what process is it used? _________________________
Translation takes place in a ________________.
__________________ and ___________________ make up ribosomes.
What is the point of translation?
Transcription and translation together is the process of ________________ _______________.
A DNA strand was sequenced using the Sanger method
(https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction
tube contained the DNA strand, fluorescently labelled
dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green,
ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA
polymerase, or its Klenow fragment. Synthesis of DNA is allowed to
proceed, and the results are shown on the right:
15
14
13
12
11
10
(a) What is the sequence of the copy and the template strands?
(b) If the template strand were in the 5'-3' direction, what will be
the sequence of the DNA copy?
Nucleotide Length
DNA Replication Terms
_____DNA polymerase III
_____Primase
_____Helicase
_____Lagging Strand
_____Leading Strand
_____RNA Primer
_____Single Strand Binding Protein
_____5’
_____3’
_____DNA Ligase
_____ Topoisomerase
_____Template Strand
_____Coding Strand
Chapter 8 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 8 - Prob. 1PCh. 8 - 8.2 In one to two sentences each, describe the...Ch. 8 - 8.3 Answer these questions concerning...Ch. 8 - 8.4 The diagram below shows a DNA duplex. The...Ch. 8 - The following is a portion of an mRNA sequence:...Ch. 8 - Compare and contrast the properties of DNA...Ch. 8 - The DNA sequences shown below are from the...Ch. 8 - Bacterial and eukaryotic gene transcripts can...Ch. 8 - Describe the two types of transcription...Ch. 8 - What is the role of enhancer sequences in...
Ch. 8 - Prob. 11PCh. 8 - Draw a bacterial promoter and label its consensus...Ch. 8 - For a eukaryotic gene whose transcription require...Ch. 8 - Three genes identified in the diagram as A, B and...Ch. 8 - Prob. 15PCh. 8 - 8.16 The segment of the bacterial gene involved in...Ch. 8 - Prob. 17PCh. 8 - Prob. 18PCh. 8 - 8.19 A DNA fragment from the end of the mouse...Ch. 8 - 8.20 Wild-type E. coli grow best at but can grow...Ch. 8 - A mutant strain of Salmonella bacteria carries a...Ch. 8 - 8.22 The human wild-type allele and a certain...Ch. 8 - Prob. 23PCh. 8 - A full-length eukaryotic gene is inserted into a...Ch. 8 - The accompanying illustration shows a portion of a...Ch. 8 - DNA footprint protection (described in Research...Ch. 8 - Suppose you have a 1-kb segment of cloned DNA that...Ch. 8 - Assume that a mutation affects the gene for each...Ch. 8 - 8.29 The DNA sequence below gives the first base...Ch. 8 - 8.30 Genomic DNA from a mouse is isolated,...Ch. 8 - 8.31 A portion of a human gene is isolated from...
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- The image shows a replication fork with template DNA strands, new DNA strands, and some replication proteins. Label the mage by moving the terms or descriptive phrases to the appropriate targets. lagging strand (or Okazaki fragment) Answer Bank protein that synthesizes RNA primer DnaB helicase DNA gyrase (topoisomerase)arrow_forwardShown is a sequencing gel. Read the sequence off the gel and determine the first 4 nucleotides (or bases) based on the fact replication occurs 5¹ to 3¹. A I G C Answer:arrow_forwardIndicate the proteins involved in the following steps of DNA replication in E. coli a. ___________ initiator of replication by binding to the origin of replication of the prokaryote ___________ a dimer that unzips the DNA helix ___________ an enzyme responsible for relieving positive supercoils ahead of replication fork ___________ maintains DNA in single-stranded state ___________ primary replicating enzyme ___________ synthesizes RNA primers The resulting gap by removal of RNA primer is filled in by ___________. h. the resulting nick is sealed by _____________.arrow_forward
- The image shows a replication fork, template DNA strands, and new DNA strands. Label the image. and 13' 15' 3' 5' Answer Bank lagging strand leading strand replication fork template DNA strandsarrow_forwardDetermine the characteristics of this piece of double-stranded DNA. GTCTTTTACTTGAAT CAGAAAATGAACTTA Phosphodiester bonds: _________________ Pyrimidines: _________________ Hydrogen bonds: _________________ Nitrogenous bases: _________________ A/T Ratio: _________________arrow_forwardName the bacterial enzyme that takes over new strand synthesis in replication after the primer is formed. Please include any number that is applicable. DNA polymerase omega DNA polymerase II DNA Polymerase epsilon DNA polymerase delta O DNA Polymerase Iarrow_forward
- The chromatogram shows fluorescent peak data from a dye-terminating nucleotide-sequencing reaction. The peaks are shown with shortest fragment on the left to longer fragments on the right. T •C A Select the DNA sequence that matches the data. 5-ТАТAСТТАСGAAGT-3' 5'-GTCCTACGGACGCG–3' 5'-ATATGAATGCTTCA–3' 5'-TGAAGCATTCATAT–3' 5-АСТТCGTAAGTATA-3'arrow_forwardA Sanger product of the sequencing of a template DNA is presented +ddTTP +ddATP +ddCTP +ddGTP Mononucleotide Pentanucleotide Trinucleotide Dinucleotide 11-nucleotide Hexanucleotide Octanucleotide Tetranucleotide 16-nucleotide Decanucleotide Nonanucleotide Heptanucleotide 17-nucleotide 15-nucleotide 13-nucleotide 12-nucleotide 18-nucleotide 20-nucleotide 14-nucleotide 19-nucleotide 21-nucleotide 1. Determine the sequence of template DNA 2. Determine the mRNA sequence from this template DNA 3. Determine the the sequence of protein product derived from that DNAarrow_forwardUse the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. There are NO sites where multiple enzymes cut. How many base pairs long is the plasmid? 3kb 2kb 4kb 5kbarrow_forward
- For each term in the left column, select the term which best matches. 1. deoxyribonucleotide 2. nitrogenous base thymine + deoxyribose 3. deoxynitronucleoside guanine + deoxyribose + phosphate group 4. polydeoxyribonucleotide DNA 5. deoxyribonucleoside 6. nitrogenous basearrow_forwardA piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane. What is it about the chemistry of DNA that causes it to be uniformly negatively charged?arrow_forwarda) Estimate the lengths of each of the DNA fragments shown in this gel A:____________ B: ____________ C: ____________ D: ____________ E: ____________ F: ____________ b) Which letter represents the DNA fragment that is the smallest? c) Which DNA fragment is approximately the same length as the lengths of fragments D & C added together? Part 4. Putting It Together 1) Consider the diagram below as well as the given information. This diagram represents a piece of circular DNA which was cut in 4 separate reactions (4 different test tubes, each with some of this DNA in it). One digest was done with AvaI, another with ClaI, a third with EcoRV, and a fourth with ScaI. The locations of the recognition sequences for each restriction enzyme are shown along with the location of that site in bp along the circle (it goes clockwise from position 1). You run an agarose gel with a molecular weight marker in the first lane, the AvaI digest in lane 2, the ClaI digest in lane 3, the…arrow_forward
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