Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 8, Problem 23P
Summary Introduction

To review:

The processes of transcription and translation as “coupled” in bacteria is described by Microbiologists. This term indicates that a bacterial mRNA can be undergoing transcription, when it is undergoing translation.

a) The coupling of possible transcription and translation in bacteria is to be described.

b) The possibility of coupling of transcription and translation in single-celled eukaryotes such as yeast is to be cheked.

Introduction:

Transcription is the process of synthesis of new RNA strands from the template one. There is a synthesis of an RNA strand from deoxy nucleotides of the sense strand. The formation of mRNA replaces the Thymine nucleotide with Uracil. RNA synthesis occurs in the direction of 5'-3' end. Any one of the DNA strands is selected for transcription. In bacteria, to save energy, the process is coupled –transcription and translation, while in eukaryotes, the process is divided and separate - transcription occurs in the nucleus and translation in the cytoplasm. In prokaryotes- the process is DNA-RNA-proteins, whereas in Eukaryotes- DNA-mRNA- RNA processing (posttranscriptional modification) - proteins.

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Students have asked these similar questions
1.) Define transcription and translation. How does transcription and translation differ in prokaryotes vs. eukaryotes (i.e. What is present in one but not the other)? 2.) What it means when we say that genetic code is redundant? 3.) Describe the stages of transcription (in detail for each step) - what components are required? 4.) Describe the stages of translation in eukaryotes (initiation, elongation, termination)
A given coding strand sequence in a Eukaryote is as follows 5'GGGAATATAA GACCGATGGA GGGTACAG CCCTATCAC GATACGCAGG ATAGCAGCA 3" a) Mark the promoter in blue and transcribe from the G after the promoter. b) Translate the mRNA made c) The mRNA made by the cell was 10 nucleotides shorter than what you have made. What could have happened? d) EXTRA practice: A particular triplet of bases in the coding strand of DNA is 5'GAC 3'. What is the amino acid for this codon and will be the anticodon on the tRNA that binds the mRNA codon?
a) What is a mutation in molecular terms?      b) a mutation deletes a base in the genomic DNA discuss how that will affect the reading frame and expression product production. Using the following list of codons describe, using diagrams etc., how information stored in the DNA is translated into a peptide. Be sure to discuss all steps. In other words, use a diagram and give me sequences, transcription and translation steps. Show the sequences of the sense and the other DNA strand, the mRNA and the tRNA’s.                    UUU -phenylalanine         UCU -serine          AUG –initiation/methionine                CUU -leucine                    ACU -threonine                   GUU -valine                      UAA -Termination

Chapter 8 Solutions

Genetic Analysis: An Integrated Approach (3rd Edition)

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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license