3000 bp 2000 bp 1000 bp 800 bp 700 bp 500 bp L Uncut EcoRI BamHI Ncol EcoR1/BamH1 BamHI/Nco1 Nco/EcoRI B/N/E

Please answer this asap. Thanks,   You have discovered a new plasmid RK21 in a unique bacterial community. As a first step towardunderstanding this plasmid, you digest the plasmid with three restriction enzymes: SspI, XhoI andSmaI. You run the digested plasmid DNA on an agarose gel, along with an uncut sample of theRK21 plasmid DNA as a control.Unfortunately you forget to load a DNA ladder, and obtain the following results. Assumecomplete digestion of all samples or all the digests worked completely

A plasmid comprised of B-DNA changed its linking number from 420 to 457, resulting in a superhelix density of 0.031. What is the length of the plasmid in base pairs? 15050 11200 3500 12532 8765

Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates?     a. The positive control would not have any bacterial lawn     b. The negative control would have as many colonies as the experimental plate   c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light     d. The experimental plate would not have any colonies   e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescent