Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 20P
The inhibitory effect of an uncompetitive inhibitor is greater at high [S] than at low [S]. Explain this observation.
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The inhibitory effect of an uncompetitive inhibitor is greater at high [S] thanat low [S]. Explain this observation.
Give an example of a noncompetitive inhibitor and its target enzyme. Draw a hypothetical Michaelis-Menten curves in the presence and absence of the noncompetitive inhibitor. Discuss the effects of noncompetitive inhibition and the reasons for these effects on the values of Km and Vmax.
Under what conditions, a higher rate in the presence of the inhibitor observed? (how could the effect of inhibitor be compensated) explain
Chapter 8 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 8 - Prob. 1PCh. 8 - The enzyme urease catalyzes the hydrolysis of urea...Ch. 8 - An enzyme contains an active site aspartic acid...Ch. 8 - The folding and unfolding rate constants for a...Ch. 8 - In some reactions, in which a protein molecule is...Ch. 8 - Would you expect an “enzyme” designed to bind to...Ch. 8 - The initial rate for an enzyme-catalyzed reaction...Ch. 8 - a. If the total enzyme concentration in Problem 7...Ch. 8 - Prob. 9PCh. 8 - Prob. 10P
Ch. 8 - The following data describe the catalysis of...Ch. 8 - At 37 oC, the serine protease subtilisin has kcat...Ch. 8 - The accompanying figure shows three...Ch. 8 - The steady-state kinetics of an enzyme are studied...Ch. 8 - The same enzyme as in Problem 14 is studied in the...Ch. 8 - Enalapril is an anti-hypertension “pro-drug"...Ch. 8 - Initial rate data for an enzyme that obeys...Ch. 8 - Prob. 18PCh. 8 - Suggest the effects of each of the following...Ch. 8 - The inhibitory effect of an uncompetitive...Ch. 8 - Prob. 21PCh. 8 - Prob. 22PCh. 8 - Prob. 23PCh. 8 - In kinetics experiments, the hydrolysis of the...
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- why is non-competitive inhibiton a special type of mixed inhibiton?arrow_forwardThe nerve gas sarin acts as a poison by covalently bonding to a hydroxyl group in the active site of the enzyme acetylcholinesterase. This binding results in a higher-than-normal amount of acetylcholine at a nerve synapse, resulting in muscle spasms. From this description, would you expect sarin to be a competitive, noncompetitive, or irreversible inhibitor? noncompetitive inhibitor irreversible inhibitor competitive inhibitorarrow_forwardAn Eadie-Hofstee plot is shown below for the different types of enzyme inhibition. Match the line withthe type of inhibition.(a) Line A Inhibition Mechanism. Ans: ________(b) Line B Inhibition Mechanism. Ans: ________(c) Line C Inhibition Mechanism. Ans: ________arrow_forward
- What is the minimum inhibitory concentration indicated in the following image?arrow_forwardWhat are the two types of enzyme inhibitors? Give anexample of each.arrow_forwardWhen we compare the binding of I and of S to the enzyme in a mixed noncompetitive inhibitor, we assumed that the binding of I decreased the affinity of the enzyme for S. What would happen if the opposite were true?arrow_forward
- describe the mechanism of each class of inhibitor, including how they impact the effective concentrations [E] and/or [ES] and where they bind on the enzyme:arrow_forwardIf the higher value of KM resulting in the new plot ( red curb ) is due to the presence of an enzyme inhibitor is inhibitor reversible or irreversible? And why?arrow_forwardUsing equilibrium argument, why does Km apparently increase, decrease or stay the same in uncompetitive inhibition?arrow_forward
- In pure noncompetitive inhibition: a. Where on the enzyme does the inhibitor bind? b. Does the inhibitor bind to E, ES or both? c. What is the effect of I on Vmax? d. What is the effect of I on Km?arrow_forwardIn concerted model, all subunits in the enzyme are either in the low affinity or high affinity forms.arrow_forwardWrite a balanced equation for the hydrolysis of cyclic GMP, catalyzed by CGMP phosphodiesterase. Would you expect an inhibitor of this enzyme to potentiate or antagonize the action of Viagra? Explain.arrow_forward
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