Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 7, Problem 16P
Summary Introduction

Introduction:

According to Chargaff’s rule, in DNA of any living organism, the ratio of nitrogen bases i.e. purine and pyrimidine is 1:1 which means number of Adenine is equal to number of Thymine (A = T) and number of Guanine is equal to number of Cytosine (G = C).

In the DNA structure Purine, Adenine (A) is always paired with pyrimidine, Thymine (T) and another purine. In the DNA structure Purine, Guanine (G) is always paired with pyrimidine, Cytosine (C).

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The melting temperature Tm of DNA can be predicted by calculation without actually measuring it. Calculate the Tm of the DNA double strand shown in (1) to (3), and discuss the results. The numbers in parentheses indicate the degree of polymerization of nucleotides.(1) A(10) + T(10), (2) A(15) + T(15), (3) G(10) + C(10)
The base composition of one of the DNA chains of a DNA double helix contains 18 mol-%A, 35 mol-%T, 26 mol-%C, and 21 mol-%G (a) What is the base composition of the complementary DNA chain? (b) Is the total amount of purine bases equal to the total amount of pyrimidine bases for the DNA double helix?
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Genetic Analysis: An Integrated Approach (3rd Edition)

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