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To analyze:
Considering the results of
Introduction:
Meselson and Stahl performed an experiment to identify the mechanism of DNA replication in any known or unknown organism. In this experiment, an organism that possessed double stranded DNA was allowed to grow on a medium containing
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Genetic Analysis: An Integrated Approach (3rd Edition)
- You are studying a microorganism that contains a chloramphenicol acetyltransferase (CAT) enzyme, and looking for a clone of this microorganism that no longer contains the gene encoding CAT. Imagine you had an LB plate containing chloramphenicol. You streak an isolated colony of the Parent Strain and Clone A onto the same plate. Which of the following statements are true about the growth pattern after 24 hours in the incubator? Select all that apply? L-hlareeheticol LB chloremphenicel Parent Clune A Parent Clone A LD chlerephenicol 18 chloremphenitol Paremt Clone A Paront Clone A Plate A is the expected growth pattern as clone A should grow in the LB plate with chloramphenicol Plate B is the expected growth pattern as LB plate with chloramphenicol parent strain should grow in the Plate B is the expected growth pattern as clone A should not grow in the LB plate with chloramphenicol Plate A is the expected growth pattern as the parent strain should not grow in the LB plate with…arrow_forwardin isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of Centrifugationarrow_forwardSuppose you have a cell-lysis sample containing genomic DNA and a complex mixture of proteins and debris. Describe different commonly used analytical methods to i) separate and purify DNA contents from other cellular contents. ii) amplify a gene of interest if present in the genomic DNA.arrow_forward
- Engineered bacteria are commonly used and cultured in pharmaceutical laboratories to produced drugs like insulin. However, during one instance, with the desire to increase the rate of insulin production, one scientist tried to modify the process and put another substance (chemical x) in the growth medium which is chemically almost the same to the substance (chemical x’) normally put in the medium. After which, the scientist found out that the amount of insulin produced in the medium decreases as compared to the normal production. Again, within the context of our discussion, explain the possible reason for the observed result.arrow_forward"the supplement that restored growth would be the molecule that the mutant strain could not synthesize" Explain this statement ?arrow_forwardIt is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?arrow_forward
- If five E. coli cells are placed into sterile nutrient media under optimal conditions (with a growth rate of 20 minutes per cell division), how many E. coli cells will be present after 6 hours of optimal growth? 5 x 26 = 320 cells 5 x 29 = 2,560 cells 5 x 212 = 20,480 cells 5 x 215 = 163,840 cells 5 x 218 = 1,310,720 cellsarrow_forwardThis research utilized a modified method of preparing activated carbons (ACs) derived from waste coconut shells loaded with silver nanoparticles (AgNP). AC was prepared through chemical impregnation method of coconut shells in H3PO4 and carbonization in a muffle furnace, while AgNP was prepared following the Turkevich method. These two substances were then combined to form silver nanoparticle-loaded activated carbon (AgAC). The samples were subjected to different characterization techniques: FTIR analysis to test the purity of the AC, FE-SEM to analyze the surface morphology of AgAC, and UV-Vis determination of AgNP formation. After characterization, the antibacterial activity of AgAC was evaluated using the Agar Well Diffusion Method. Results show that AgNPs were observed to form at an average maximum absorbance of 2.063 at a wavelength interval of 421-423 nm. It was also observed that the synthesized AC exhibited the FTIR result comparable to the commercially-available AC. Further,…arrow_forwardAssume the DpnI digestion has failed after the PCR in the synthesis of mutated plasmid by thermal cycling process. Under such circumstance, a) What kind of transformants would be found on the plate? Provide reason(s) for your answer. b) How would it affect the number of colonies that contain the mutant construct? Provide reason(s) for your answer. asaparrow_forward
- For each of the following ( A & B ) provide the method of transfer and a brief explanation as to why the method would not take place under the conditions described . 1. Which method of DNA transfer between bacteria would not take place if the donor and recipient were separated by a filter with a pore size of 0.45 um or another physical barrier 2. Which method of transfer would be blocked by the presence of high concentrations of DNAase ( enzymes capable of degrading DNA ) ?arrow_forward"Hybridization of a single-stranded DNA molecule attached to a fluorophore with a preparation of metaphase chromosomes that have been partially denatured" is a description of which laboratory method?arrow_forwardWould you expect transfer of chromosomal DNA by conjugation to be more efficient if cells were plated together on solid medium (agar) or mixed together in a liquid in a shaking flask? Explain.arrow_forward
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