Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 6, Problem 9P
How is the frequency of cotransduction related to the relative positions of genes on a bacterial chromosome? Draw a map of three genes and describe the expected relationship of cotransduction frequencies to the map.
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DNA from a strain of Bacillus subtilis with genotype a + b + c + d + e + is used to transform a strain with genotype a – b – c – d – e – . Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? Show how you work out the order by diagraming.
DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a– b– c– d– e–. Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? please show how you worked out the order by diagraming, thank you!
Pair of genes Co-transformation
a+ and b+ no
a+ and c+ no
a+ and d+ yes
a+ and e+ yes
b+ and c+ yes
b+ and d+ no
b+ and e+ no
c+ and d+ no
c+ and e+ yes
d+ and e+ no
In a transformation experiment involving a wild type bacterial strain with a recipient strain with mutations in genes f,
g, h and i pairs of genes were analyzed for co-transformation with the following results:
Gene Pair
Co-transformation
g+ i+
f+ i+
yes
no
f+ h+
Уes
f+ g+
no
g+ h+
h+ i+
no
уes
What is the linear order of these genes relative to cach other?
Chapter 6 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- If you mated an F' bacterial cell with lacl+ lacP+ lacO+ lacZ+ lacY+ on the bacterial chromosome and lacl+ lacP+ lacOc lacZ+ lacY- on a plasmid with an F- lacl+ lacP+ lacO+ lacZ+ lacY+ and lactose was absent and glucose was absent, what expression levels do you predict in the RECIPENT cell after conjugation (assume no recombination)? Betagalactosidase expression will be HIGH Betagalactosidase expression will be BASAL Betagalactosidase expression will be LOWER THAN BASAL There will be NO Betagalactosidase expressionarrow_forwardLet’s suppose a new strain of P1 phage has been identified thatpackages larger pieces of the E. coli chromosome. This P1 strainpackages pieces of the E. coli chromosome that are 5 minuteslong. If two genes are 0.7 minute apart along the E. coli chromosome,what would be the cotransduction frequency using a normalstrain of P1 and using the new strain of P1 that packages largerpieces? What would be the experimental advantage of using thisnew P1 strain?arrow_forwardYou have a strain of Neurospora that is unable to synthesize histidine and thus requires H in the media in order to grow. You have isolated one revertant colony. Predict the expected proportion of the progeny that would be h+ if you cross the colony with the original mutant colony and the reversion occurred by each of the following mechanisms: Precise change of the mutated base back to its original base. A suppressor gene is mutated on a different chromosome A suppressor gene is mutated on the same chromosome but 10mu distant from the mutated gene. The mutant colony is crossed to a wild-type Neurospora colony and the following data are collected. 95% of all asci scored are h+ but 5% are h-. Which mechanism in part a is consistent with these data? Explain why and what has happened on a molecular level.arrow_forward
- How does homologous recombination with transfected disruption constructs can inactivate specific target genes in yeast? Explain this with the help of figure.arrow_forwardDNA from a strain of Bacillus subtilis with the genotype trp+ tyr+ was used to transform a recipient strain with the genotype trp− tyr−. The following numbers of transformed cells were recovered: Genotype Number of transformed cells trp+ tyr− 154 trp− tyr+ 312 trp+ tyr+ 354 What do these results suggest about the linkage of the trp and tyr genes?arrow_forwardA bacterial strain with genotype his− leu− thr− is transformed with DNA from a strain that is his+ leu+ thr+. A few leu+ thr+ cells and a few his+ thr+ cells are found, but no his+ leu+ cells are observed. Which genes are farthest apart?arrow_forward
- A donor strain of bacteria with genotype leu+ gal− pro+ is infected with phages. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are leu− gal+ pro−. The second strain is selected for leu+, and the following cotransduction data are obtained: Donor Recipient Selected allele Cells with cotransduced allele (%) leu+ gal− pro+ leu− gal+ pro− leu+ 47 pro+ leu+ 26 gal− Which genes are closest, leu and gal or leu and pro?arrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:Draw a map that shows the order of genes and the locations ofthe origins of transfer among these different Hfr strains?arrow_forwardThe presence (+) or absence (−) of six sequences in each of five bacterial artificial chromosome (BAC) clones (A–E) is indicated in the following table. Using these markers, put the BAC clones in their correct order and indicate the locations of the numbered sequences within them.arrow_forward
- A generalized transduction e xperiment uses a metE+pyrD+ strain as donor and metE- pyrD- as recipient.metE+ transductants are selected and then tested for thepyrD+ allele. The following numbers were obtained:metE+ pyrD- 857metE+ pyrD+ 1Do these results suggest that these loci are closelylinked? What other explanations are there for the lone“double”arrow_forwardA donor strain of bacteria with alleles a+ b+ c+ is infected with phages to map the donor chromosome using generalized transduction. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are a− b− c−. Bacteria with the a+ allele are selected, and the percentage of cells with cotransduced b+ and c+ alleles are recorded. Donor Recipient Selected allele Cells with cotransduced allele (%) a+ b+ c+ a− b− c− a+ 25 b+ a+ 3 c+ Is gene b or gene c closer to gene a? Explain your reasoning.arrow_forwardCrosses are carried out between a bacterial F- strain and three different Hfr strains. In these strains, it takes 60 minutes for an Hfr strain to transfer the entire chromosome. Below are the data generated from interrupted mating experiments. Time of transfer of different genes (in minutes) is given for each strain. Appearance of genes in F- cells Hfr1: Genes b+ d+ c+ f+ g+ Time 3 5 16 27 59 Hfr2: Genes e+ f+ c+ d+ b+ Time 6 24 35 46 48 Hfr3 Genes d+ c+ f+ e+ g+ Time 4 15 26 44 58 Based on these data, you could conclude that Group of answer choices b is between g and d and is 4 minutes from g e is between f and g and is 25 minutes from g g is between e and b and is 24 minutes from e none of these is correct d is between b and c and is 10 minutes from barrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license