Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 6, Problem 10P
Describe the differences between genetic complementation and recombination as they relate to the detection of
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Describe the reason behind the occurrence of phage growth and release by the mating with the non-lysogenic recipient, but not with the infection by a lysogenic recipient.
Consider the phase variation of flagella in Salmonella. Describe the phenotype of a cell in which hin is deleted. Will it be motile? Will it undergo phase variation? Which flagellin(s) will be used, if any? Name two bacterial defenses against bacteriophages and explain how they work How might a phage evolve resistance to the CRISPR host defense?
Shown below are the complementation test results involving 4 independently isolated lethal mutants in a bacteriophage. Complementation was assayed by simultaneouly infecting bacteria with two phage strains, each with a different mutation, neither of which could alone lyse the cells. In the table below, a "+" indicates the strains complemented each other and therefore lysed open the bacteria. A "0" indicates no complementation and therefore no cell lysis occurred.
Test pair Results 1___2___3___4
1,2 + 1 0 + + 0
1,3 + 2 0 + +
1,4 0 3 0 +
2,3 + 4 0
2,4 +
3,4 +
How many genes are there?
a. 3
b.1
c. 2
d. 4
Chapter 6 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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- Draw and label the E. coli host cell/T4 bacteriophage interaction.?arrow_forwardIn 1944, Avery, Macleod, and McCarty provided strong evidence that DNA is the hereditary material in Streptococcus pneumoniae by Group of answer choices showing that avirulent cells could become virulent by the process of transduction none of these is true. showing that virulent cells could become avirulent if the DNA was destroyed after transformation showing that avirulent cells could not gain the ability to become virulent cells if conjugation was interrupted. showing that avirulent cells could not gain the ability to become virulent if DNA was destroyed after transformation.arrow_forwardEight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by wild-type bacteriophages. The mutant strains were allowed to infect the bacteria in a complementation test. A +' indicates that lysis occurred with coinfection. Aindicates that lysis did not occur. GKWTMAGC +++ +++ - -+ -| ++ + ++ + - + + M -|- +1+ A + + A cistron is defined by no complementation in the : configuration. How many genes are controlling lysis in this bacteriophage? (Use a number not a word in the space) Which of the following strains below are defective in the same gene as strain Q? Answer yes if the strain is defective or no if it is not. Strain Defective K. W A. N/Aarrow_forward
- What is meant by the term site-specific recombination as used in identifying the processes that lead to the integration of temperate bacteriophages into host bacterial chromosomes during lysogeny or to the formation of specialized transducing phage?arrow_forwardV/D/J recombination frequently yields a gene whose mRNA cannot be successfully translated into an immunoglobulin chain. What aspect of somatic recombination is likely to produce nonproductive gene rearrangement?arrow_forwardwhen various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid plaques. Explain the difference between the two types of plaques. can all bacteriophage form clear and turbid plaques?arrow_forward
- Bacteriophage P22 was used in generalised transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the recipient strains of S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the selected for one of the three genetic markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the other two markers. markers. The results are given in the following table: Strain I donor str thrA aceA thrA str aceA+ Strain recipient strs thrA+ aceA thrA str aceA Phenotype selected Str Ace+ Str recombinants selected ThrA ThrA ThrA ThrA Ace Ace Number 60 40 95 5 10 90 str: gene involved in streptomycin resistance, aceA: gene involved in the use of acetate as a carbon source, thrA: gene involved in threonine biosynthesis. 1) What are the selective media used in these three transduction experiments? to obtain the selected…arrow_forwardEight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by wild-type bacteriophages. The mutant strains were allowed to infect the bacteria in a complementation test. A "+" indicates that lysis occurred with coinfection. A "-" indicates that lysis did not occur. GKWTMAQC G- ++++- K W T M A Q C - + +++ - ++++ - + + - + - + - - + + + A cistron is defined by no complementation in the How many genes are controlling lysis in this bacteriophage? (Use a number not a word in the space) configuration.arrow_forwardRegarding the production of monoclonal antibodies, it is CORRECT to state that: * A) Hybridomas are cells generated from the fusion of myeloma cells and cells from animals challenged with the antigen of interest that have the ability to produce antibodies and limited cell proliferation. B) All hybrid cells generated from the fusion of cells from the challenged animal and cells of the myeloma lineage will have the ability to produce antibodies reactive against the antigen of interest. C) The HAT selection medium contains aminopterin, which is an efficient inhibitor of the thymidine kinase metabolic pathway, thus eliminating myeloma cells that are not fused or fused with other cells of the same kind. D) The HAT selection medium contains hypoxanthine, which is an efficient inhibitor of the nucleotide synthesis salvage pathway, thus eliminating cells from the challenged animal that are not fused or fused with other identical cells. E) Hybridomas will produce antibodies with the…arrow_forward
- During the analysis of seven rII mutations in phage T4, mutants1, 2, and 6 were in cistron A, while mutants 3, 4, and 5 were incistron B. Of these, mutant 4 was a deletion overlapping mutant5. The remainder were point mutations. Nothing was knownabout mutant 7. Predict the results of complementation (+ or -)between 1 and 2; 1 and 3; 2 and 4; and 4 and 5.arrow_forwardThe Pfizer Covid-19 vaccine is one of the vaccines currently being rolled out for mass vaccination in South Africa to protect the population against SARS CoV-2, and the active compound in this vaccine is an mRNA molecule. a) The starting material for productio of this vaccine is a copy of the coronavirus spike protein gene cloned into a ppasmid. List the main experimental steps in the vaccine production process, to go from a plamid to an mRNA molecule. b) Which molecular processes in the cell will allow an injected strand of mRNA to produce anti-Covid immunity in the vaccine recipient?arrow_forwardTargeting vectors use homologoues recombination to insert transgenes at specific chosen locations in the host genome. Discuss this statement.arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license