Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Question
Chapter 6, Problem 26P
Summary Introduction
To analyze:
To devise an experiment to identify Auxotrophic bacteria that are unable to produce lysine (
Introduction:
Replica plating, a simple technique to transfer few cells from each bacterial colony in a plate to another plate, will be useful to identify auxotrophic bacteria in a pool of prototrophic bacteria.
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The synthesis of arginine by Nuerospora was determined by examining a number of
mutant strains that were unable to synthesize the compound. Use the table of
bacterial growth below to 1) determine the correct sequence of the synthesis pathway
and 2) where in the synthesis pathway each mutation interrupts the synthesis. A “+"
indicates growth.
Nothing
added to
Succinate
Ornithine
added
Strain
Cirtulline
Arginine
Added
added
added
growth
medium
Wild
Mutant 1
Mutant 2
Mutant 3
Mutant 4
A series of auxotrophic mutants were isolated in Neurospora. Examination
of fungi containing these mutations revealed that they grew on minimal
medium to which various compounds (A, B, C, D) were added; growth
responses to each of the four compounds are presented in the following
table. Give the order of compounds A, B, C, and D in a biochemical
pathway. Outline a biochemical pathway that includes these four
compounds and indicate which step in the pathway is affected by each of
the mutations.
Compound
Mutation number
А в С
A
D
134
276
987
773
772
146
333
123
+ + I +
You have several different media onto which you inoculated eight strains of yeast (A-H). The media
include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented
with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin.
After overnight incubation, the following results were observed (tan patches represent growth):
D
plate 1
(A) B
DE F
GH
plate 5
plate 4
plate 6
Which plate contains an unsupplemented minimal medium? [Select]
Which plate contains a rich medium? [Select]
plate 2
Which strain is a prototroph? [Select]
Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy?
[ Select]
plate 3
plate 7
One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to
be? [Select]
Chapter 6 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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