Order of Transfer of Several Different Hfr Bacterial Genes Strain Origin First Last Н thr leu azi ton pro lac gal str met leu thr met str gal lac pro ton azi pro ton azi leu thr met str gal lac 3 lac pro ton azi leu thr met gal str 4 met str gal lac pro ton azi leu thr met thr leu azi ton pro lac gal str met thr leu azi ton pro lac gal str ton azi leu thr met str gal lac pro

Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.

You are a graduate student working to construct a single gene knockout library of Leptospiria kirschneri, one the causative agents of leptospirosis. You are looking for single gene mutants which disrupt the bacterium’s spirillum shape to determine what role this rare cellular morphology may play in disease development and progression. Using an appropriate donor strain, you introduce the plasmid shown into L. kirschneri. L. kirschneri is not able to replicate the plasmid. The repeat regions are denoted on the plasmid map as vertical black lines, the transposase is denoted as tnp, and kanamycin kinase is denoted as aph. The larger of the two regions is transposed. Following selection and counter-selection, you isolate several non-spirillum colonies, which you use to infect juvenile piglets. Most of the infected piglets develop leptospirosis. Isolating L. kirschneri from these animals reveals that it has regained its spirillum morphology. What is a likely explanation for this reversion of…

An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.Make a calculation.

The following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a d'g recipient. at dt g+= 84% ad g+= 6% at dgt = 10% ad g+ = less than 1% Which gene is in the middle? a The genes are located too far apart on the bacterial genome to determine which gene is in the middle. d Insufficient data are provided to answer this question.