Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 5P
Explain the importance of the following features in conjugating donor bacteria:
a. the origin of transfer
b. the conjugation pilus
c. homologous recombination
d. the relaxosome
e. relaxase
f. T strand DNA
g. pilin protein
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Some strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply.
A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor
B. transformation resulted in expression of a virulence factor
C. horizontal gene transfer between different species of bacteria
D. transduction of a specific gene resulted in expression of new traits
For bacteria that are F+, Hfr, F', and F- answer the following.
a. Describe the state of the F factor.
b. Which of these cells are donors? Which is the recipient?
c. Which of these donors can convert exconjugants to a donor state?
d. Which of these donors can transfer a donor gene to exconjugants?
e. Describe the results of conjugation (i.e., changes in the recipient and the exconjugant) that allow detection of the state of the F factor in a donor strain.
f. Describe a "partial diploid" and how it originates.
Define the following terms:a. transpositionb. transposable elementc. bacterial transformationd. transductione. conjugation
Chapter 6 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A hypothetical gene for cephalosporin resistance is found to be carried by a transposon. Explain what a transposon is. Then explain how the cephalosporin resistance could be horizontally transferred between organisms by transformation, conjugation, and transduction. What steps/events would have to occur to allow the transposon to be transferred by each method. Also, explain how it could be transferred vertically between organisms.arrow_forwardGenetic information can be transferred horizontally from one bacterial cell to another cell of the "same generation" (versus daughter cells), leading to genetic recombination, by all of the following EXCEPT: A. Gene expression (transcription/translation/protein synthesis) B. Transformation C. Conjugation D. Transduction E. None of the other four answers (all are mechanisms of “horizontal transfer” genetic recombination)arrow_forwardDefine the following terms: a. nonreplicative transposition b. transposable element c. bacterial transformation d. transduction e. conjugationarrow_forward
- The likelihood of co-transformation of two bacterial genes increases: a. As their distance apart on the chromosome increases b. As their distance apart on the chromosome decreases c. As the transducing phage enter the lytic cycle d. As recombination rate decreases e. None of these is correcarrow_forwardThe following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a d'g recipient. at dt g+= 84% ad g+= 6% at dg+ = 10% ad g+= less than 1% What is the map distance between the a and d genes? 6 map units less than 1 map unit 10 map units 84 map units 74 map unitsarrow_forwardWhich of the following statement about conjugation is TRUE: During F+ to F- mating, the donor becomes F-. During the Hfr to F- transfer, the recipient becomes F+. The whole bacteria genome may get transferred during Hfr mating. During the Hfr to F- transfer, some recipients become recombinants in which transferred fragment recombines with homologous region in the genome.arrow_forward
- Explain how a U-tube apparatus can distinguish between genetic transfer involving conjugation and genetic transfer involving transduction. Do you think a U-tube could be used to distinguish between transduction and transformation?arrow_forwardBelow is a diagram of the general structure of the bacteriophagel chromosome. Speculate on the mechanism by which it forms aclosed ring upon infection of the host cell. 5'GGGCGGCGACCT:double@stranded region-3' 3'- double@stranded region:CCCGCCGCTGGA5'arrow_forwardDefine the following terms: a. transfection b. replicative transposition c. composite transposition d. retrotransposon e. insertional elementarrow_forward
- Describe the events (in words) occurring during conjugation in bacterial cells (using F plasmid transfer)arrow_forwardReferring to conjugation in bacteria: Mark all cell types for which the following statement is true. Cell is used as a donor cell in conjugation. Select one or more: O a. F+ O b. F- O c. Hfr O d. F"arrow_forwardDefine the following terms: a. lytic cycle b. prophage c. transduction d. lysogeny e. nuclocapsidarrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license