Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 5, Problem 5.13PS
(a)
Summary Introduction
To determine: The reaction for the phosphorylation of glucose by the transfer of phosphate group from ATP, along with
Introduction: In a system, multiple
(b)
Summary Introduction
To determine: The reaction for the transfer of phosphate from phosphocreatine to ADP to generate creatine and ATP, and
Introduction: In a system, multiple chemical reactions can take place at same time. Each of the proceeding reactions has their own thermodynamic parameters such as enthalpy, free energy and others. If these reactions take place in a sequential manner and are a part of a bigger chemical process, then the total free energy for the complete system can be calculated using the free energies of the individual reactions.
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ENZYME KINETICS ANALYSIS
of 6
Xanthine oxidase (XO) is the enzyme that catalyzes the synthesis of uric acid, which in excess
causes gouty arthritis. The inhibition of this enzyme is therefore critical in its treatment. A student
researcher is investigating the inhibitory effects of kaempferol (Kmp) and chlorogenic acid (Cha) on XO
which uses xanthine (Xan) as substrate. Table 1 below shows the enzyme kinetic data. Construct the
Lineweaver-Burk plot complete with the linear regression analvsis. Fill in the needed information on Table
2 and paste a copy of your Lineweaver-Burk plot. submit the picture of your output in PNG or JPG format.
Table 1. Enzyme Kinetic Data
Velocity, mM/s
[S], mM
Хan
Kmp
Cha
0.492
0.0678
0.0351
0.0615
0.211
0.0531
0.0261
0.0451
0.087
0.0298
0.0157
0.0211
0.048
0.0195
0.0091
0.0142
0.029
0.0127
0.0067
0.0081
Table 2. Enzyme Kinetic Parameters
Xanthine
Kaempferol
Chlorogenic acid
Parameters
Vmax
Км
Type of Inhibition
Mode of Binding
NA
NA
Lineweaver-Burk Plot
Chapter 5 Solutions
Becker's World of the Cell (9th Edition)
Ch. 5 - How do phototrophs and chemotrophs depend...Ch. 5 - How does the spontaneity of a chemical reaction...Ch. 5 - QUESTION: Why does the height of the spike get...Ch. 5 - For a chemical reaction happening in a cell, what...Ch. 5 - Prob. 5.1PSCh. 5 - QUANTITATIVE Photosynthetic Energy Transduction....Ch. 5 - Energy Conversion. Most cellular activities...Ch. 5 - Problem Set Enthalpy, Entropy, and Free Energy....Ch. 5 - Violating the Second Law? The second law of...Ch. 5 - QUANTITATIVE The Equilibrium Constant. The...
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