Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 4, Problem 4TQ
Summary Introduction
To review:
The doubling time in case an exponentially growing culture has an optical density (OD) at 600
nanometers (nm)Â (OD600) of 0.2 after 30 minutes and OD600 of 0.8 after 80 minutes.
Introduction:
The microbial cells undergo various types of reproduction such as binary fission, multiple fission, and budding. The bacterial cells can be grown on a culture medium under laboratory conditions. The growth pattern exhibited by dividing bacterial cells in a batch culture is exponential consisting of
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h, calculate g, n, and k forthis culture.
A culture of S. cerevisea has an overnight OD of 2.3
(1.0 OD is approx 1.0x107 cells/ml)
You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies.
How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ul
If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium?
how to calculate these kind of question in tissue culture media preperation
Chapter 4 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 4.1 - Prob. 1TQCh. 4.2 - Prob. 1TQCh. 4.2 - Prob. 2TQCh. 4.3 - Prob. 1TQCh. 4.3 - Prob. 2TQCh. 4.3 - Prob. 3TQCh. 4.3 - Prob. 4TQCh. 4.4 - Prob. 1TQCh. 4.4 - Prob. 2TQCh. 4.4 - Prob. 3TQ
Ch. 4.4 - Prob. 4TQCh. 4.4 - Prob. 5TQCh. 4.4 - Prob. 6TQCh. 4.4 - Prob. 7TQCh. 4.4 - Prob. 8TQCh. 4.6 - Prob. 1TQCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQCh. 4 - Prob. 9RQCh. 4 - Prob. 1TQCh. 4 - Prob. 2TQCh. 4 - Prob. 3TQCh. 4 - Prob. 4TQCh. 4 - Prob. 5TQCh. 4 - Prob. 6TQCh. 4 - Prob. 7TQ
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- if 250 colonies are present on a pour plate made from10^5 dilution, how many colonies should grow on a plate made from 1:10^6 dilution? Explain.arrow_forwardvolume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h. (answer:P = 67,55 g/L)arrow_forwardDescribe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution using only 4.5 mL diluents in tubes. What would be the theoretical cell count if 0.1 mL of the 2nd and 4th dilutions were each plated?arrow_forward
- If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original culturearrow_forwardAssume you have a stock culture at 5 x 109 cells/mL and you wish to inoculate 1 liter of fresh medium so that in 15 hours the cell density will be 2 x 108/mL. Assume a generation time of 3.5 hours. What should be the dilution?arrow_forwardYou wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you needto centrifuge the culture at 2,000xg for 10 minutes at room temperature. The rotor of yourcentrifuge has a maximum diameter of 25.4cm and the minimum diameter of 12.4cm. At whatrpm should you spin the centrifuge for pelleting the cells?arrow_forward
- Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?arrow_forwardAssume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?arrow_forwardBased on prior experiments, you have determined that for a culture of S. aureus an absorbance reading of 1.0 AU650 is approximately equal to 7.25 x 106 cells/mL.How many cells should be present after 10 generations in a 1 mL culture, if the initial cell culture had an absorbance reading of 0.5? (3 Points)arrow_forward
- If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramarrow_forwardStarting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.arrow_forwardThe culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Principles Of Radiographic Imaging: An Art And A ...Health & NutritionISBN:9781337711067Author:Richard R. Carlton, Arlene M. Adler, Vesna BalacPublisher:Cengage Learning
Principles Of Radiographic Imaging: An Art And A ...
Health & Nutrition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Cengage Learning
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY