The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?

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The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?

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Introduction

A serial dilution is a process of sequential dilution in order to decrease the density of cell culture. It is associated with a dilution factor. 

A factor that dilutes the stock solution to the desired concentration is the dilution factor (DF). DF is expressed by the ratio of the final volume (diluted solution) to the initial volume (undiluted solution).

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