If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagram

Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?

When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?

B) A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold with dimensions of 20mm x 20mm x 4mm at a final density of 2 x 104 cells/mm³. After culturing the harvested cells, you found that they have a doubling time of 36 hours. Determine the total number of cells (N) needed to fill the scaffold. Show your work.

How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?  suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.

N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?

You are growing a culture of S. aureus. You know that 9 generations have occurred after 6 hours. What is the generation time in minutes? if i started a culture with 1000 cells, after 8 generations, how many cells will i have?

B) A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold with dimensions of 20mm x 20mm x 4mm at a final density of 2 x 104 cells/mm³. After culturing the harvested cells, you found that they have a doubling time of 36 hours. Given the growth rate is proportional to the number of cells, determine the time it will take to achieve the desired cell count. Show your work.

Select all that apply to a negative stain:    1. involves a washing step 2. cells may be distorted or shrunken  3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells

You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix.  You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water.  What is the dilution of the bacterial culture in bottle B?