Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 4.4, Problem 2TQ
Summary Introduction
To analyze:
The time taken to fall ill in the provided scenario.
Introduction:
There are various factors that must be taken into consideration while calculating the microbial population in different conditions. Each type of species of the microorganism takes a different time in order to become double or produce offspring. The microorganisms undergo division through various processes such as binary fission, budding, and multiple fission.
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A media contains 2 E. coli cells which have a generation time and a replication time of 60 and 40 mins respectively. How many cells will be produced after 5 hours?
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A bacterium synthesizes DNA at each replication fork at a rate of 1500 nucleotides per second. If this bacterium completely replicates its circular chromosome by theta replication in 20 minutes, how many base pairs of DNA will its chromosome contain?
Chapter 4 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 4.1 - Prob. 1TQCh. 4.2 - Prob. 1TQCh. 4.2 - Prob. 2TQCh. 4.3 - Prob. 1TQCh. 4.3 - Prob. 2TQCh. 4.3 - Prob. 3TQCh. 4.3 - Prob. 4TQCh. 4.4 - Prob. 1TQCh. 4.4 - Prob. 2TQCh. 4.4 - Prob. 3TQ
Ch. 4.4 - Prob. 4TQCh. 4.4 - Prob. 5TQCh. 4.4 - Prob. 6TQCh. 4.4 - Prob. 7TQCh. 4.4 - Prob. 8TQCh. 4.6 - Prob. 1TQCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQCh. 4 - Prob. 9RQCh. 4 - Prob. 1TQCh. 4 - Prob. 2TQCh. 4 - Prob. 3TQCh. 4 - Prob. 4TQCh. 4 - Prob. 5TQCh. 4 - Prob. 6TQCh. 4 - Prob. 7TQ
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- The proteins and enzymes listed below are all required for DNA replication in E. coli, but they are listed in a random order. Determine the correct order in which they function in replication, by selecting the correct number from the drop-down menu in each case, with 1 being first and 6 being last.arrow_forwardThere are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).arrow_forwardIn eukaryotes, the DNA replication rate is 50 nucleotides per second. How long would the replication of a chromosome of 150 million base pairs take if eukaryotic chromosomes were replicated like those of prokaryotes? Actually, eukaryotic replication takes only several hours. How do eukaryotes achieve this high rate?arrow_forward
- Using 14N isotope medium for DNA replication instead of 15N, what would be observed ifDNA replication were conservative in one cycle of replication/in three cycles? How aboutdispersive replication in one cycle, in three cycles?arrow_forwardThe E. coli chromosome is 1.28 mm long. Under optimal conditions, thechromosome is replicated in 40 minutes.(a) What is the distance traversed by one replication fork in 1 minute?(b) If replicating DNA is in the B form (10.4 base pairs per turn), how manynucleotides are incorporated in 1 minute in one replication fork?(c) If cultured human cells (such as HeLa cells) replicate 1.2 m of DNAduring a five-hour S phase and at a rate of fork movement one-tenthof that seen in E. coli, how many origins of replication must the cellscontain?(d) What is the average distance, in kilobase pairs, between these origins?arrow_forwardSuppose that replication is initiated in a medium containing moderately radioactive tritiated thymine. After a few minutes of incubation, the bacteria are transferred to a medium containing highly radioactive tritiated thymidine. Sketch the autoradiographic pattern that would be seen for (a) undirectional replication and (b) bidirectional replication, each from a single origin.arrow_forward
- You decide to repeat the Meselson-Stahl experiment, except this time you plan to grow the E. coli cells on light 14N medium for many generations and then transfer them to heavy 15N medium and allow them to grow for 2 additional generations (2 rounds of DNA replication). If the conservative model of DNA replication was correct, what is the expected distribution of DNA in the density gradient after two rounds of replication?arrow_forwardExplain the following statement : a) initiation of bacteriall DNA replication is an energy requiring process b) bacterial DNA polymerase can enter the termination sequence but cannot exisarrow_forwardIf deoxyribonucleotides that lack the 3’-OH groups are added during the replication process, what do you expect will occur? Describe what happens when a nonsense mutation is introduced into the gene encoding transposase within a transposon. A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colony morphology is strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology?arrow_forward
- "In a replication bubble, the same parental DNA strand serves as the template strand for leading strand synthesis in one replication fork and as the template for lagging-strand synthesis in the other fork" is true or false.arrow_forwarda) Under normal conditions E. coli produces three DNA polymerases. State their functional similarities and differences. b) List the other proteins and enzymes involved in DNA replication in E.coli and give their functions.arrow_forwardIt takes 40 minutes to completely replicate the E. coli chromosome, even in an optimally nourished cell. However, bacterial cells can divide as frequently as every 20 minutes. How can cells divide more rapidly, apparently, than their DNA can be copied?arrow_forward
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