Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 4, Problem 3TQ
Summary Introduction
To analyze:
The tube containing higher number of bacteria in the given conditions after 2 and 4 hours.
Introduction:
Bacteria are grown in laboratory conditions in a culture medium. This medium is composed of all the essential nutrients that are required for the growth of bacterial cells. The composition of media can be modified in order to selectively grow a particular type of bacteria and then, the media is referred to as selective media.
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Suppose 1,000 bacteria are inoculated into a tube of a minimal salt medium where they double once an hour and 10 cells are inoculated into a rich medium, where they double every 20 minutes. Which tube will have more cells in 2 hours? In 4 hours?
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Chapter 4 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 4.1 - Prob. 1TQCh. 4.2 - Prob. 1TQCh. 4.2 - Prob. 2TQCh. 4.3 - Prob. 1TQCh. 4.3 - Prob. 2TQCh. 4.3 - Prob. 3TQCh. 4.3 - Prob. 4TQCh. 4.4 - Prob. 1TQCh. 4.4 - Prob. 2TQCh. 4.4 - Prob. 3TQ
Ch. 4.4 - Prob. 4TQCh. 4.4 - Prob. 5TQCh. 4.4 - Prob. 6TQCh. 4.4 - Prob. 7TQCh. 4.4 - Prob. 8TQCh. 4.6 - Prob. 1TQCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQCh. 4 - Prob. 9RQCh. 4 - Prob. 1TQCh. 4 - Prob. 2TQCh. 4 - Prob. 3TQCh. 4 - Prob. 4TQCh. 4 - Prob. 5TQCh. 4 - Prob. 6TQCh. 4 - Prob. 7TQ
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- If a bacterium has a generation time of 30 minutes, and you start with 100 cells at time 0, how many cells will you have in 30, 60, 90, and 120 minutes?arrow_forwardGiven a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?arrow_forwardA species of bacteria has a doubling time of 24 hours. After incubating the initial population for one week, the final population was 6,400 cells. What was the initial population of that bacteria? Show all work. Equations: Nt = No x 2n Final concentration = (initial concentration)/(total dilution factor) Total dilution factor = (individual dilution factor)# of serial dilutionsarrow_forward
- Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xoarrow_forwardThe number of bacteria in culture increases rapidly. The table below gives the number of bacteria a few times (in hours) after the moment when. (a) Find the average rate of change for the number of bacteria from 0 hours to 3.1 hours. (b) Find the average rate of change for the number of bacteria from 6.2 hours to 15.5 hours.arrow_forwardSuppose you count 35 bacterial cells from a solution that has gone through the following serial dilutions: two 10-fold dilutions, followed by a 5-fold dilution, followed by 2-fold dilution. What should be the bacterial count from the original solution? Show your workarrow_forward
- If a single 1.7 × 10−10 m3 bacterium could reproduce through cell division at a rate of 3(t − 0.5t), where t is measured in minutes, how much volume (in m3) will the resulting bacteria colony occupy after 1 hour? bacteria colony volume = _____ × 104 m3arrow_forwardA bacterium has a generation time of 30 minutes. You transfer cells from an exponentially growing culture to a fresh source of the same medium so that the freshly inoculated medium contains 3.2 x 106 cells per milliliter. This new culture does not exhibit a lag phase. About how many cells (per milliliter) should you have after 1.5 hours incubation?? Solve this step by step and by using formulaarrow_forwarda) Draw the diagram and describe motility required for the bacteria that have flagella to an attractant (light)? b) Draw the diagram of the two-chamber microbial fuel cell under continuous operation and label completely.arrow_forward
- A slant is inoculated with 100 E.coli cells. The slant is left at room temperature where the generation time is 1 hour. How many E. coli are on the slant in 6 hours? If the slant was initially put in the 37C incubator, with a 20 minute generation time, how many more cells would have grown in the same time period?arrow_forwardYou inoculate two test tubes with the same amount of identical growth medium and with the same number of identical yeast cells and grow these cells under identical conditions except for the presence or absence of oxygen. a) After 12 hours, all of the glucose in each culture has been consumed. You determine the number of total cells found in each culture and find that one culture has more cells than the other. Which culture would have the greater cell density, the one grown aerobically or the one grown anaerobically? Explain why the culture you chose above can make more cells with the same amount of glucose than the other culture.arrow_forwardIn a certain culture of bacteria, the rate of increase is proportional to the number present. (a) If it is found that the number doubles in 6 hrs, how many may be expected at the end of 18 hrs? (b) If there are 102 at the end of 4 hrs, and 8 ∙ 102 at the end of 8 hrs, how many were there in the beginning?arrow_forward
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