You wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you need to centrifuge the culture at 2,000xg for 10 minutes at room temperature. The rotor of your centrifuge has a maximum diameter of 25.4cm and the minimum diameter of 12.4cm. At what rpm should you spin the centrifuge for pelleting the cells?

B) A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold with dimensions of 20mm x 20mm x 4mm at a final density of 2 x 104 cells/mm³. After culturing the harvested cells, you found that they have a doubling time of 36 hours. Given the growth rate is proportional to the number of cells, determine the time it will take to achieve the desired cell count. Show your work.

In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar plate? in the agar directly below the disc O in the agar halfway between the disc and the petri dish wall O in the agar furthest away from the disc the cehmical is equally distributed throughout the agar

wwwwww The total number of cells in a culture is counted using dye trypan blue and is found to be 3.8 x 106 cells/mL. The culture is diluted 1:22 and then 200μl seeded per well into a 96 well plate. What is the final cell density per well? ssibility: Good to go MacBook Air