Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 4.3, Problem 2TQ
Summary Introduction

To review:

The effect of lack of lactose from the MacConkey medium on the growth of lactose-fermenting Escherichia coli bacteria.

Introduction:

The bacteria are broadly classified into the two categories, namely the gram-positive and gram-negative bacteria. The gram positive bacteria are stained positively with the gram stain, while the gram-negative bacteria do not take up the gram stain. These bacteria require different growth factors.

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In your laboratory, you have an F− strain of E. coli that is resistantto streptomycin and is unable to metabolize lactose, but it can metabolizeglucose. Therefore, this strain can grow on a medium thatcontains glucose and streptomycin, but it cannot grow on a mediumcontaining lactose. A researcher has sent you two E. colistrains in two separate tubes. One strain, let’s call it strain A, hasan F′ factor (an F prime factor) that carries the genes that are requiredfor lactose metabolism. On its chromosome, it also has thegenes that are required for glucose metabolism. However, it is sensitiveto streptomycin. This strain can grow on a medium containinglactose or glucose, but it cannot grow if streptomycin is addedto the medium. The second strain, let’s call it strain B, is an F−strain. On its chromosome, it has the genes that are required forlactose and glucose metabolism. Strain B is also sensitive to streptomycin.Unfortunately, when strains A and B were sent to you, thelabels had fallen…
TRUE OF FALSE: Is E coli colonies would be pink mucoid in Eosin Methylene BlueAgar (EMBA)? Is Coliform colonies would be pink mucoid in Eosin Methylene BlueAgar (EMBA)?
Strain X. spp. is a rod-shaped, anaerobic, Gram-positive bacterium with peritrichous flagella andpili that could use glucose and dye as its sole carbon source. Answer the following sections (a, band c).a) With a doubling time of 240 minutes and a starting population size of 10 bacteria cells. Howmany bacteria cells will be present after 72 hours, assuming no cell death?b) Draw the diagram and describe motility required for the bacteria to gradient concentrationof attractant (food)?
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