Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 31, Problem 21P
Interpretation Introduction
Interpretation:
An affinity-labeling reagent for the tRNA binding sites in E. coli ribosomes should be determined.
Concept introduction:
The inhibitor that formsa covalent bond with its target (basically an enzyme) and inactivates it, is termed as the affinity label. The basic mechanism of the affinity label is to specifically and reversible deliver a weak reactive group to enzyme that can irreversibly binds to an amino acid residue.
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tRNA enzyme. Any given aminoacyl-tRNA synthetase: a. Attaches the amino acid to the 5′-end '5end of the tRNA b. Always recognizes only one specific tRNA c. Recognizes all tRNA molecules d. Forms an ester linkage between the amino acid and the tRNA
Be sure to answer all parts.
Write a possible mRNA sequence that codes for each peptide.
a. His-Cys-Tyr-Val-Ser
5¹-
b. Phe-Val-Thr-Tyr-Glu
5'-
5'-
c. Trp-Phe-Asn-Gln
-3'
U
-3'
с
Table 26.2 The Genetic Code-Triplets in Messenger RNA
First Base
(5' end)
-3'
U
UUU
UUC
UUA
UUG
CUU
CUC
CUA
CUG
AUL
Phe
Phe
Leu
Leu
Leu
Leu
Leu
Leu
la
C
UCU
UCC
UCA
UCG
CCU
CCC
CCA
CCG
Second Base
A
UAU
UAC
UAA
UAG
CAU
CAC
CAA
CAG
Ser
Ser
Ser
Ser
Pro
Pro
Pro
Pro
Tyr
55
Tyr
Stop
Stop
His
His
Gin
Gin
G
UGU
UGC
UGA
UGG
CGU
CGC
CGA
CGG
Cys
Cys
Stop
Trp
Arg
Arg
Arg
Arg
Third Base
(3¹ ond)
DUAC DU AG
с
А
А
Transcription. Using strand 1 of the DNA molecule as a template, transcribe a messenger RNA molecule (a.k.a. mRNA transcript).
Strand 1
3’ End
TTG
CTT
CAC
CTT
GCG
CGC
CCG
CGC
TAA
TTG
5’ end
mRNA
Chapter 31 Solutions
Biochemistry
Ch. 31 - Prob. 1PCh. 31 - Prob. 2PCh. 31 - Prob. 3PCh. 31 - Prob. 4PCh. 31 - Prob. 5PCh. 31 - Prob. 6PCh. 31 - Prob. 7PCh. 31 - Prob. 8PCh. 31 - Prob. 9PCh. 31 - Prob. 10P
Ch. 31 - Prob. 11PCh. 31 - Prob. 12PCh. 31 - Prob. 13PCh. 31 - Prob. 14PCh. 31 - Prob. 15PCh. 31 - Prob. 16PCh. 31 - Prob. 17PCh. 31 - Prob. 18PCh. 31 - Prob. 19PCh. 31 - Prob. 20PCh. 31 - Prob. 21PCh. 31 - Prob. 22PCh. 31 - Prob. 23PCh. 31 - Prob. 24PCh. 31 - Prob. 25PCh. 31 - Prob. 26PCh. 31 - Prob. 27PCh. 31 - Prob. 28PCh. 31 - Prob. 29PCh. 31 - Prob. 30PCh. 31 - Prob. 31PCh. 31 - Prob. 32PCh. 31 - Prob. 33PCh. 31 - Prob. 34PCh. 31 - Prob. 35PCh. 31 - Prob. 36PCh. 31 - Prob. 37PCh. 31 - Prob. 38PCh. 31 - Prob. 39PCh. 31 - Prob. 40PCh. 31 - Prob. 41PCh. 31 - Prob. 42PCh. 31 - Prob. 43PCh. 31 - Prob. 44PCh. 31 - Prob. 45PCh. 31 - Prob. 46PCh. 31 - Prob. 47PCh. 31 - Prob. 48PCh. 31 - Prob. 49PCh. 31 - Prob. 50PCh. 31 - Prob. 51PCh. 31 - Prob. 52P
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