Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Question
Chapter 3, Problem 3CTQ
a.
Summary Introduction
To determine:
The type of microscope that is used to get the best image in the given scenario
Concept introduction:
The microscopes are used to study the structure of microbes and cells of various living organisms. In light microscopes, the light of visible range is used to observe the specimen.
b.
Summary Introduction
To determine:
The type of microscope that is used to get the best image in the given scenario
Concept introduction:
Beam of electrons is used in electron microscopy. The magnets are used to refract the electron beam. The beam passes through the specimen. A very thin slice of specimen is observed under electron microscopes.
c.
Summary Introduction
To determine:
The type of microscope that is used to get the best image in the given scenario
Concept introduction:
Electron microscopes are used to study the cellular structure of the organisms. A beam of electrons is used in electron microscopes. The image produced from electron microscope is black and white because there is no use of natural light.
d.
Summary Introduction
To determine:
The type of microscope that is used to get the best image in the given scenario
Concept introduction:
Prions are the small infectious particles that are made up of disease causing proteins. Abnormal folding of proteins is found in prions. Prions can cause neurological disorders in human beings.
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Students have asked these similar questions
a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained)
b. What is the microscopy technique and magnification used to obtain this image?
c. What is the basic principle of image formation using this microscopy technique?
d. What can be observed and concluded from the image of the specimen?
e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.
1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions.
1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?
a. Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image below.
b. Suppose the corresponding Gram negative control were purple. Explain what step(s) of the Gram stain you would adjust and how you would adjust them to ensure the accuracy of your Unknown results
Chapter 3 Solutions
Microbiology: A Systems Approach
Ch. 3.1 - Explain what the Five Is mean and what each step...Ch. 3.1 - Discuss three physical states of media and when...Ch. 3.1 - Compare and contrast selective and differential...Ch. 3.1 - Provide brief definitions for defined and complex...Ch. 3.2 - Prob. 5AYPCh. 3.2 - Prob. 6AYPCh. 3.2 - Prob. 7AYPCh. 3.2 - Prob. 8AYPCh. 3.2 - Compare and contrast the three main categories of...Ch. 3 - Prob. 1MCQ
Ch. 3 - A mixed culture is a. the same as a contaminated...Ch. 3 - Resolution is ____ with a longer wavelength of...Ch. 3 - A real image is produced by the a. ocular. b....Ch. 3 - Prob. 5MCQCh. 3 - The specimen for an electron microscope is always...Ch. 3 - Prob. 7MCQCh. 3 - Bacteria tend to stain more readily with cationic...Ch. 3 - Multiple Matching. For each type of medium, select...Ch. 3 - A fastidious organism must be grown on what type...Ch. 3 - Agar has the disadvantage of being easily...Ch. 3 - A subculture is a culture made from an isolated...Ch. 3 - Prob. 13TFCh. 3 - Prob. 14TFCh. 3 - The best stain to use to visualize a microorganism...Ch. 3 - What is the functional type of mannitol salt agar...Ch. 3 - Create a short paragraph to differentiate among...Ch. 3 - Prob. 3CTQCh. 3 - a.Create a paragraph to differentiate among the...Ch. 3 - You are a scientist studying a marsh area...Ch. 3 - Visual Connections Blooms Level 5: Evaluate These...Ch. 3 - Prob. 1CM
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- Which of the following describes the usefulness of a Gram stain. Select all that apply. a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used. c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.arrow_forwardWhich of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texturearrow_forwarda. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?arrow_forward
- The resolution of a microscope is affected by the wavelength of light used to examine the sample. O 1) True O 2) Falsearrow_forwardExplain the difference between Gram positive and Gram negative cell walls. Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?) How is the Gram stain used clinically to help identify the cause of an infection?arrow_forwardA microbiology student was given a mixed culture of two different gram positive bacteria species to grow into a culture medium using correct aseptic techniques. After two days,one gram positive bacterial species grew on the media and the growth appeared red on the surface of the medium. Tthe second gram positive bacterial species grew and the growth appeared yellow on the surface of the medium. What is the possible explanation for the differences in the color of the bacterial growth? A. the culture was contaminated B. the microbiologist put too much inoculum on the culture medium C. the medium was a selective medium D. the medium was differentialarrow_forward
- Comparing the different methods for counting bacterial cells, which of the following is FALSE? A. The serial dilution methods counts only viable cells. B. The turbidity method indirectly measures the number of cells observing how much light goes through a broth sample C. The turbidity method uses a spectrophotometer D. The direct microscopic method counts only viable cells E. The direct microscopic method counts both viable and non-viable cellsarrow_forwardAttached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? (see rubrics for the point marking) A before washing hands C 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.arrow_forwardWhich type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infectionarrow_forward
- Three-dimensional images of specimens can be produced using z stacks obtained from confocal microscopes. O 1) True O 2) Falsearrow_forwardWhich of the following is NOT correctly matched? A. Halophile – microbe that can grow in an environment with high osmotic pressure (e.g., salt) B. None of the other four answers (all are correctly matched) C. Agar – complex polysaccharide from seaweed used as a solidifying agent in culture media D. Colony – a population of microbial cells arising from a single cell or spore or from a group of attached cells E. Capnophile – microbe requiring an elevated molecular oxygen (O2) environment for optimal growtharrow_forwardAssume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.arrow_forward
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