Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 3, Problem 7MCQ
Summary Introduction
Introduction:
The cells which are being observed under optical microscopes are suspended in broth, water, or saline. The suitable medium helps in maintaining the viability of cells for a temporary time and the medium provides space to the cells for movement.
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the purpose of a mordant in Gram stain is
a.
to prevent the crystal violet from leaving the cell
b.
to make bacteria cell larger
c.
to fix iodine inside the cell
d.
to remove simple stain
The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain staining and capsule staining. What result would you expect to see?
a. none of these answers
b. gram positive, endospore negative, acid-fast negative, capsule positive
c. gram negative, endospore positive, acid-fast positive, capsule negative
d. gram positive, endospore positive, acid-fast negative, capsule positive
e. gram negative, endospore positive, acid-fast negative, capsule negative
thank you!!
Give at least one disease that can be diagnosed by: a. Gram Stain b. Acid-fast stain c. Endospore stain d. Capsule stain e. Flagella Stain
Chapter 3 Solutions
Microbiology: A Systems Approach
Ch. 3.1 - Explain what the Five Is mean and what each step...Ch. 3.1 - Discuss three physical states of media and when...Ch. 3.1 - Compare and contrast selective and differential...Ch. 3.1 - Provide brief definitions for defined and complex...Ch. 3.2 - Prob. 5AYPCh. 3.2 - Prob. 6AYPCh. 3.2 - Prob. 7AYPCh. 3.2 - Prob. 8AYPCh. 3.2 - Compare and contrast the three main categories of...Ch. 3 - Prob. 1MCQ
Ch. 3 - A mixed culture is a. the same as a contaminated...Ch. 3 - Resolution is ____ with a longer wavelength of...Ch. 3 - A real image is produced by the a. ocular. b....Ch. 3 - Prob. 5MCQCh. 3 - The specimen for an electron microscope is always...Ch. 3 - Prob. 7MCQCh. 3 - Bacteria tend to stain more readily with cationic...Ch. 3 - Multiple Matching. For each type of medium, select...Ch. 3 - A fastidious organism must be grown on what type...Ch. 3 - Agar has the disadvantage of being easily...Ch. 3 - A subculture is a culture made from an isolated...Ch. 3 - Prob. 13TFCh. 3 - Prob. 14TFCh. 3 - The best stain to use to visualize a microorganism...Ch. 3 - What is the functional type of mannitol salt agar...Ch. 3 - Create a short paragraph to differentiate among...Ch. 3 - Prob. 3CTQCh. 3 - a.Create a paragraph to differentiate among the...Ch. 3 - You are a scientist studying a marsh area...Ch. 3 - Visual Connections Blooms Level 5: Evaluate These...Ch. 3 - Prob. 1CM
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- observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the abovearrow_forwardWhich of the following WILL INCREASE gelling if addition of Ca2+ ions was added? a. starch b. alginates c. agar d. chitinarrow_forwardThe primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. Safraninearrow_forward
- Crystal violet ( choose all that apply) A. Is not a good differential stain B. Will stain the cytoplasm of the cell walls C. Can be used to stain peptidoglycan on bacteria cell wall D. Can be used as an effective antimicrobialarrow_forwardThe Gram stain, if the decolorizing step is deleted, gram-negative cells will appear at the completion of the staining procedure. 4. a. Purple b. diaphragm С. Micrometer d. 0.02-AUM ribosomearrow_forwardWhich of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texturearrow_forward
- Which of the following is not an aim of fixation? A. To prevent putrefaction B. Remove excess water from the tissue C. Prevent osmotic swelling D. Render the tissue suitable for subsequent stainingarrow_forwardGive 3 disease (each) that can be diagnosed by: a. Gram Stain b. Acid-fast stain c. Endospore stain d. Capsule stain e. Flagella Stainarrow_forwardWhat will happen if you FAIL to do the following during bacterial cell staining.a. Smear Preparationb. Heat fixingc. Acid fuchsin d. Steaming of cells with endosporee. Mordantarrow_forward
- Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C. Ethanol D. Crystal Violetarrow_forwardWhy are staining methods used in microbiology? Choose all that apply A. Staining results can have medical implications for an infected B. To clearly see the internal structures of the bacteria C. The stains can be used to dinginuish between bacteria type D. It is easier to very small microscope.arrow_forwardExplain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before stainingarrow_forward
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