Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 3, Problem 1CTQ
What is the functional type of mannitol salt agar (MSA)?
Explain how it is utilized in the isolation and identification of Staphylococcus species.
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Chapter 3 Solutions
Microbiology: A Systems Approach
Ch. 3.1 - Explain what the Five Is mean and what each step...Ch. 3.1 - Discuss three physical states of media and when...Ch. 3.1 - Compare and contrast selective and differential...Ch. 3.1 - Provide brief definitions for defined and complex...Ch. 3.2 - Prob. 5AYPCh. 3.2 - Prob. 6AYPCh. 3.2 - Prob. 7AYPCh. 3.2 - Prob. 8AYPCh. 3.2 - Compare and contrast the three main categories of...Ch. 3 - Prob. 1MCQ
Ch. 3 - A mixed culture is a. the same as a contaminated...Ch. 3 - Resolution is ____ with a longer wavelength of...Ch. 3 - A real image is produced by the a. ocular. b....Ch. 3 - Prob. 5MCQCh. 3 - The specimen for an electron microscope is always...Ch. 3 - Prob. 7MCQCh. 3 - Bacteria tend to stain more readily with cationic...Ch. 3 - Multiple Matching. For each type of medium, select...Ch. 3 - A fastidious organism must be grown on what type...Ch. 3 - Agar has the disadvantage of being easily...Ch. 3 - A subculture is a culture made from an isolated...Ch. 3 - Prob. 13TFCh. 3 - Prob. 14TFCh. 3 - The best stain to use to visualize a microorganism...Ch. 3 - What is the functional type of mannitol salt agar...Ch. 3 - Create a short paragraph to differentiate among...Ch. 3 - Prob. 3CTQCh. 3 - a.Create a paragraph to differentiate among the...Ch. 3 - You are a scientist studying a marsh area...Ch. 3 - Visual Connections Blooms Level 5: Evaluate These...Ch. 3 - Prob. 1CM
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- Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agararrow_forwardWhat are the possible problems/limitations to be encountered in a laboratory experiment about the Isolation of Foodborne Pathogens on Selective, Differential, and Enriched media (e.g., blood agar, mcconkey, mannitol, XLT-4) using steak plating method. Provide a brief but concise explanation for every limitations, respectively.arrow_forwardDescribe the selective and differential properties of mannitol salt agar (MSA) for the isolation and identification of staphylococci.arrow_forward
- Vibrio cholerae can be cultured on Thiosulfate-Citrate-Bile salts-Sucrose agar (TCBS). Enterobacteriaceae and gram-positive bacteria are inhibited on this agar. cholerae produces large yellow colonies while other Vibrio species produce blue, green, or blue-green colonies. Discuss the purpose of each media listed. Based on this information alone, what term(s) would best describe this agar? Selective Enrichment (enriched) Differential Reducing Selective and enriched Selective and differential Selective, differential, and enrichedarrow_forwardNot only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate? Multiple Choice a) Gamma-hemolytic b) Alpha-hemolytic c) Non-hemolytic d) Beta-hemolyticarrow_forwardDescribe how specimen collection is performed using aseptic techniques to isolate medically significant bacteria and identify these clinically important bacterial species, Escherichia coli, Streptococcus pneumonia, Haemophilus influenzae and Streptococcus pyogenes by using different staining techniques and biochemical tests. Evaluate the effectiveness of different biochemical techniques used to identify different bacterial species.arrow_forward
- You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures ,State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminatedarrow_forwardWhy is glucose not the choice of carbohydrate for most differential media used in the identification of gram (-) enteric bacilli?arrow_forwardWhat is it called when you stab the nutrient agar during the inoculation step of isolating a microorganism?arrow_forward
- Based on the journal article “Interpretation of the Tube Coagulase Test for Identification of Staphylococcus aureus”, what should be the result to conclude the organism is S.aureus? When is it necessary to have supplemental tests done for the identification of S.aureus?arrow_forwardMannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?arrow_forwardWhat are the purposes of the antimicrobial agents present in the selectivemedia used for the isolation of Campylobacter?arrow_forward
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