Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Question
Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope.
c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred
Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.
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