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Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope.
c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred
Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.
Step by step
Solved in 3 steps
- a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.D) When performing fluorescence microscopy what are the stokes shift and why is it better to have fluorochromes with a large stokes shift? E) What is photobleaching and what is done when imaging histological samples to overcome it when performing fluorescence microscopy?What is phase contrast? Give examples of phase contrast in optical microscopy.
- a) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FMa) Briefly describe the concept of and instrument configuration for confocal microscopy. b) How do confocal and conventional microscopy compare? c) What other microscopy techniques can provide super-resolution?Answer the following questions: Why are most cells so small? Why cell size is limited? List two instances when the coarse adjustment knob is never used Why is immersion oil used with the 100X objective? List two common problems associated with using the microscope and how you would go about solving it. When should the lenses be cleaned? What is the correct way to clean them?
- How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityWhen focusing a light microscope, why is it best to adjust the focus using the coarse focusing knob before using the fine focusing knob?The third micrograph is at the lowest magnification. Identify A (name of the cells; blank 1). Identify B (name of the structures arrows point to; blank 2) - Identify C (name of the structures arrows point to; blank 3) High mag low mag high mag Blank # 1 Blank # 2 Blank # 3
- Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?a. Why must you slowly turn the fine adjustment knob? b. Is natural light or is artificial light used when using the microscope?What is a wet mount technique in microscopy?