Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 3, Problem 1VC
Visual Connections Bloom’s Level 5: Evaluate
These questions use visual images or previous content to make connections to this chapter’s concepts.
1. Process Figure 3.10. If you were using the streak plate method to plate a very dilute broth culture (with many fewer bacteria than the broth used here) would you expect to see single, isolated colonies in area 4 or area 3? Explain your answer.
Note: This method only works if the spreading tool (using an inoculating loop) is resterilized after each of steps 1-4.
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A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colonymorphology is strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology?
Suppose that you were tasked with 4 cultures of a specific Bacteria Species in Luria Broth medium: (1) culture A – cells are in lag phase; (2) culture B – cell in log phase; (3) culture C – cells in stationary phase; and culture D – cells in decline phase. Imagine that you observe the growth rates of each culture in a fresh sterilized LB medium. Now, plot the outcome growth curves of cultures A, B, C and D in a single graph.
Solve the following parts.
a. What phase of the bacterial growth curve does a chemostat maintain?
b. Define the term generation time?
c. What is a culture medium?
Chapter 3 Solutions
Microbiology: A Systems Approach
Ch. 3.1 - Explain what the Five Is mean and what each step...Ch. 3.1 - Discuss three physical states of media and when...Ch. 3.1 - Compare and contrast selective and differential...Ch. 3.1 - Provide brief definitions for defined and complex...Ch. 3.2 - Prob. 5AYPCh. 3.2 - Prob. 6AYPCh. 3.2 - Prob. 7AYPCh. 3.2 - Prob. 8AYPCh. 3.2 - Compare and contrast the three main categories of...Ch. 3 - Prob. 1MCQ
Ch. 3 - A mixed culture is a. the same as a contaminated...Ch. 3 - Resolution is ____ with a longer wavelength of...Ch. 3 - A real image is produced by the a. ocular. b....Ch. 3 - Prob. 5MCQCh. 3 - The specimen for an electron microscope is always...Ch. 3 - Prob. 7MCQCh. 3 - Bacteria tend to stain more readily with cationic...Ch. 3 - Multiple Matching. For each type of medium, select...Ch. 3 - A fastidious organism must be grown on what type...Ch. 3 - Agar has the disadvantage of being easily...Ch. 3 - A subculture is a culture made from an isolated...Ch. 3 - Prob. 13TFCh. 3 - Prob. 14TFCh. 3 - The best stain to use to visualize a microorganism...Ch. 3 - What is the functional type of mannitol salt agar...Ch. 3 - Create a short paragraph to differentiate among...Ch. 3 - Prob. 3CTQCh. 3 - a.Create a paragraph to differentiate among the...Ch. 3 - You are a scientist studying a marsh area...Ch. 3 - Visual Connections Blooms Level 5: Evaluate These...Ch. 3 - Prob. 1CM
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