Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 3, Problem 4CTQ
a. Create a paragraph to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain.
b. For each of the following scenarios, explain which staining technique(s) should be used.
- analyzing cell wall composition
- observing structures for locomotion
- identifying a structure enhancing pathogenicity
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a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained)
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Chapter 3 Solutions
Microbiology: A Systems Approach
Ch. 3.1 - Explain what the Five Is mean and what each step...Ch. 3.1 - Discuss three physical states of media and when...Ch. 3.1 - Compare and contrast selective and differential...Ch. 3.1 - Provide brief definitions for defined and complex...Ch. 3.2 - Prob. 5AYPCh. 3.2 - Prob. 6AYPCh. 3.2 - Prob. 7AYPCh. 3.2 - Prob. 8AYPCh. 3.2 - Compare and contrast the three main categories of...Ch. 3 - Prob. 1MCQ
Ch. 3 - A mixed culture is a. the same as a contaminated...Ch. 3 - Resolution is ____ with a longer wavelength of...Ch. 3 - A real image is produced by the a. ocular. b....Ch. 3 - Prob. 5MCQCh. 3 - The specimen for an electron microscope is always...Ch. 3 - Prob. 7MCQCh. 3 - Bacteria tend to stain more readily with cationic...Ch. 3 - Multiple Matching. For each type of medium, select...Ch. 3 - A fastidious organism must be grown on what type...Ch. 3 - Agar has the disadvantage of being easily...Ch. 3 - A subculture is a culture made from an isolated...Ch. 3 - Prob. 13TFCh. 3 - Prob. 14TFCh. 3 - The best stain to use to visualize a microorganism...Ch. 3 - What is the functional type of mannitol salt agar...Ch. 3 - Create a short paragraph to differentiate among...Ch. 3 - Prob. 3CTQCh. 3 - a.Create a paragraph to differentiate among the...Ch. 3 - You are a scientist studying a marsh area...Ch. 3 - Visual Connections Blooms Level 5: Evaluate These...Ch. 3 - Prob. 1CM
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- a. Explain the purposes of tissue fixation and tissue staining. b. Identify the factors that affect the quality, of tissue fixation and describe the effects of fixation on tissue (e.g., Temperature, size of tissue, time of fixation, or osmolality of fixative.) c. Describe the following types of microscopes and describe reasons for their use. a. Light (compound microscopy), b Polarizing microscopy, c. Phase-contrast, d. Interference microscopy, e. Darkfield, f. Fluorescence, g. Electron (scanning and transmission). h. Confocal microscope. d.…arrow_forwardDiscuss the steps from dissection of tissue to producing a section on a slide ready for staining, as provided for this practical and as would be carried out in a hospital lab. include principles and procedures for each step. maximum word limit 400arrow_forwardIdentify: 1. Cell shape and arrangement in A? 2. Gram stain reaction in A? 3. Cell shape and arrangement in B? 4. Gram stain reaction in B? 5. Genus and species of B? (component of the normal flora of the skin)arrow_forward
- MICROBIOLOGY: Microscopic Morphology of Microbes Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content). another: What is the advantage of the Gram stain over the simple stain? What is the theory about the mechanism of the Gram-stain reaction?arrow_forwardPlease discuss why staining is important in visualizing microbes under the microscope. What different types of staining methods can be used?arrow_forwardDefine the term gram staining.arrow_forward
- a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.arrow_forwardComplete the table below. Specify the simple stain used, the cell morphology, and a fun fact about the organism.arrow_forwardDescribe the steps of the Gram stain, and explain how it can be animportant diagnostic tool for infections.arrow_forward
- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedarrow_forwardanswer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:arrow_forwardSelect all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cellsarrow_forward
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