Concept explainers
(a)
Interpretation:
The reason by which cell C can grow in the HAT medium is to be determined.
Concept introduction:
HAT medium is the selection medium, used for mammalian cell culture. It is the combination of hypoxanthine, aminopterin, and thymidine. Hypoxanthine and thymidine are the intermediates formed in the DNA synthesis. While aminopterin is the inhibitor of dihydrofolate reductase.
(b)
Interpretation:
Distinction between the normal cells A and the cells that have taken up the foreign DNA is to be determined.
Concept introduction:
HAT medium is the selection medium, used for mammalian cell culture. It is the combination of hypoxanthine, aminopterin, and thymidine. Hypoxanthine and thymidine are the intermediates formed in the DNA synthesis. While aminopterin is the inhibitor of dihydrofolate reductase.
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Biochemistry
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