Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question
Chapter 25, Problem 30P
Interpretation Introduction
Interpretation:
The labeled nitrogen in IMP is to be determined.
Concept introduction:
Purine is a heterocyclic ring, which is composed of a pyrimidine ring fused with an imidazole ring. Adenine and guanine found in DNA and RNA are purines. It is oxidized into uric acid by the action of xanthine oxidase. Further, it is also involved in the formation of certain alkaloids, such as caffeine, and theophylline.
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On the trail of carbons. Tissue culture cells were incubated with
glutamine labeled with 15NN in the amide group. Subsequently,
IMP was isolated and found to contain some 15N.N. Which atoms
in IMP were labeled?
Cyclic forms of D-glucose: Haworth formula
The furanose and pyranose forms of D-glucose can be presented using Haworth formula. The following
templates are used for this formula. Note the numbering of the carbon atoms and the anomeric carbon
(this carbon defines the alpha and beta isomer).
Furanose template (furan)
Pyranose template (pyran)
anomeric carbon
anomeric carbon
Compare the Fisher and Haworth formula of D-glucofuranose and D-glucopyranose. Notice that the
groups attached to C-1, C-2, and C-3 on the left side of the Fisher formula are written above the plane
of the furanose template.
Fisher formula
Haworth formula
H FOH
H-2
HOH,č 5
-OH
FOH-
H
HO-3H
4
он
H
1
H-4
H-5
H
OH
3
6 CH2OH
H
OH
Alpha-D-glucofuranose
Alpha-D-glucofuranose
HOH2C-
он
OH
но
H
FOH-
OH
Но
H
H.
OH
H
ČH2OH
Beta-D-glucofuranose
Beta-D-glucofuranose
More ratios. Through the use of nuclear magnetic resonance
spectroscopy, it is possible to determine the ratio between the
protonated and deprotonated forms of buffers. (a) Suppose the ratio
of [ A- ]A I to [HA] is determined to be 0.1 for a buffer with
pKar6.0.pKa = 6.0. What is the pH? (b) For a different buffer,
91974
suppose the ratio of [ A- ]lA J to [HA] is determined to be 0.1 and
the pHpH is 7.0. In this case, what is the pKapKa of the buffer? (c) For
another buffer with pKa=7.5PKa = 7.5 at pH 8.0pH 8.0, what is the
expected ratio of [ A- ][A ] to [HA]? do
Chapter 25 Solutions
Biochemistry
Ch. 25 - Prob. 1PCh. 25 - Prob. 2PCh. 25 - Prob. 3PCh. 25 - Prob. 4PCh. 25 - Prob. 5PCh. 25 - Prob. 6PCh. 25 - Prob. 7PCh. 25 - Prob. 8PCh. 25 - Prob. 9PCh. 25 - Prob. 10P
Ch. 25 - Prob. 11PCh. 25 - Prob. 12PCh. 25 - Prob. 13PCh. 25 - Prob. 14PCh. 25 - Prob. 15PCh. 25 - Prob. 16PCh. 25 - Prob. 17PCh. 25 - Prob. 18PCh. 25 - Prob. 19PCh. 25 - Prob. 20PCh. 25 - Prob. 21PCh. 25 - Prob. 22PCh. 25 - Prob. 23PCh. 25 - Prob. 24PCh. 25 - Prob. 25PCh. 25 - Prob. 26PCh. 25 - Prob. 27PCh. 25 - Prob. 28PCh. 25 - Prob. 29PCh. 25 - Prob. 30PCh. 25 - Prob. 31PCh. 25 - Prob. 32PCh. 25 - Prob. 33PCh. 25 - Prob. 34PCh. 25 - Prob. 35PCh. 25 - Prob. 36PCh. 25 - Prob. 37PCh. 25 - Prob. 38PCh. 25 - Prob. 39PCh. 25 - Prob. 40PCh. 25 - Prob. 41PCh. 25 - Prob. 42PCh. 25 - Prob. 43PCh. 25 - Prob. 44PCh. 25 - Prob. 45PCh. 25 - Prob. 46PCh. 25 - Prob. 47PCh. 25 - Prob. 48PCh. 25 - Prob. 49PCh. 25 - Prob. 50PCh. 25 - Prob. 51P
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