Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
expand_more
expand_more
format_list_bulleted
Question
Chapter 25, Problem 49P
Interpretation Introduction
(a)
Interpretation:
Results in the graph are to be determined.
Concept introduction:
Lesch-Nyhan syndrome is an X-linked inherited disorder. It affects about 380,000 people across the globe. Due to this uric acid gets accumulated in the blood and urine, leading to hyperuricemia and hyperuricosuria. This also leads to gout and kidney problems.
Interpretation Introduction
(b)
Interpretation:
The reason for the Lasch-Nyhan syndrome in LND1 person is to be determined.
Concept introduction:
Lesch-Nyhan syndrome is an X-linked inherited disorder. It affects about 380,000 people across the globe. Due to this uric acid gets accumulated in the blood and urine, leading to hyperuricemia and hyperuricosuria. This also leads to gout and kidney problems.
Interpretation Introduction
(c)
Interpretation:
Explanation for the graph B is to be determined.
Concept introduction:
Lesch-Nyhan syndrome is an X-linked inherited disorder. It affects about 380,000 people across the globe. Due to this uric acid gets accumulated in the blood and urine, leading to hyperuricemia and hyperuricosuria. This also leads to gout and kidney problems.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Catalase is the enzyme. experiment with h2O2
3. What is the marker for the enzyme’s optimum condition?
Need help
Sesamin is one of the major lignans found in sesame oil. Sesamin-metabolic pathways remain uncharacterized at both the enzyme and gene levels. However, one microorganism showed significant sesamin-degrading activity and was identified as Sinomonas sp. no. 22. A sesamin- metabolizing enzyme named SesA was purified from this strain and characterized. Explain how sesamin methylene transferase was identified and characterized and explain the mechanism. Based on your knowledge, what other assays could be run to characterize this enzyme?
The enzymatic activity of PFK1 is generally measured by set- ting up a coupled enzyme assay system whereby aldolase, triose phos- phate isomerase, and glycerol-3-phosphate dehydrogenase are added to the assay mixture. For the latter enzyme, NADH is added and its change in concentration is readily monitored at 340 nm.
Write the chain of reactions catalyzed by these enzymes using structural formulas, label substrates and products, and explain why the coupled en- zyme assay system leads to oxidation of NADH. While the chain of reac- tions is similar to those in glycolysis, there is a critical difference because of the dehydrogenase enzyme. Describe how this enzyme causes the chain of reactions to differ from those in glycolysis.
Chapter 25 Solutions
Biochemistry
Ch. 25 - Prob. 1PCh. 25 - Prob. 2PCh. 25 - Prob. 3PCh. 25 - Prob. 4PCh. 25 - Prob. 5PCh. 25 - Prob. 6PCh. 25 - Prob. 7PCh. 25 - Prob. 8PCh. 25 - Prob. 9PCh. 25 - Prob. 10P
Ch. 25 - Prob. 11PCh. 25 - Prob. 12PCh. 25 - Prob. 13PCh. 25 - Prob. 14PCh. 25 - Prob. 15PCh. 25 - Prob. 16PCh. 25 - Prob. 17PCh. 25 - Prob. 18PCh. 25 - Prob. 19PCh. 25 - Prob. 20PCh. 25 - Prob. 21PCh. 25 - Prob. 22PCh. 25 - Prob. 23PCh. 25 - Prob. 24PCh. 25 - Prob. 25PCh. 25 - Prob. 26PCh. 25 - Prob. 27PCh. 25 - Prob. 28PCh. 25 - Prob. 29PCh. 25 - Prob. 30PCh. 25 - Prob. 31PCh. 25 - Prob. 32PCh. 25 - Prob. 33PCh. 25 - Prob. 34PCh. 25 - Prob. 35PCh. 25 - Prob. 36PCh. 25 - Prob. 37PCh. 25 - Prob. 38PCh. 25 - Prob. 39PCh. 25 - Prob. 40PCh. 25 - Prob. 41PCh. 25 - Prob. 42PCh. 25 - Prob. 43PCh. 25 - Prob. 44PCh. 25 - Prob. 45PCh. 25 - Prob. 46PCh. 25 - Prob. 47PCh. 25 - Prob. 48PCh. 25 - Prob. 49PCh. 25 - Prob. 50PCh. 25 - Prob. 51P
Knowledge Booster
Similar questions
- Intracellular and extracellular enzymes require different extraction methods. Provide 2 examples of intracellular enzyme extraction methods and explain the reason for performing this step.arrow_forward47. No exchange. Mice that completely lack ATP-ADP translocase (ANT- /ANT - ) can be made by using the knockout technique. Remarkably, these mice are viable but have the following pathological conditions: (1) high serum levels of lactate, alanine, and succinate; (2) little electron transport; and (3) a six- to eightfold increase in the level of mitochondrial H2O2 compared with that in normal mice. Provide a possible biochemical explanation for each of these conditions.arrow_forward*The enzyme glucose oxidase isolated from the mold Penicillium notatum catalyzes the oxidation of 3-D-glucose to D-glucono-6- lactose. This enzyme is highly specific for the ß anomer of In spite of this glucose and does not affect the a anomer. specificity, the reaction catalyzed by glucose oxidase is commonly used in a clinical assay for total blood glucose that is, for solutions consisting of a mixture of 3- and a-D- glucose. What are the circumstances required to make this possible? Aside from allowing the detection of smaller quan- tities of glucose, what advantage does glucose oxidase offer over non-enzymatic oxidizing agents like Tollens reagent? *Is B-D-glucosamine a reducing sugar?arrow_forward
- Using the ActiveModel for aldose reductase, describe the structure of the TIM barrel motif and the structure and location of the active site.arrow_forwardNormal cells die in a nutrient medium containing thymidine and methotrexate, whereas mutant cells defective in thymidylate synthase survive and grow. Explain.arrow_forwardWhat is the catalytic efficiency of Catalase ? Table. The values of KM and kcat for some Enzymes and Substrates Enzyme Carbonic anhydrase Substrate CO2 HCO3 KM (M) 1.2 x 10-2 2.6 x 10-2 Kcat (s-1) 1.0 x 106 4.0 x 105 Catalase H2O2 2.5 x 10-2 1.0 x 107 Urease Urea 2.5 x 10-2 4.0 x 105 O A. 4 x 108 M-s-1 O B. 4 x 108 M-1.s-1 OC25x 10-9 M-s1 D. 2.5 x 102 M-1.s-1 OE 1.0 x 107 s1arrow_forward
- PLP can catalyze both \alpha, \beta elimination reactions and \beta, \gamma - elimination reactions. Propose a mechanism for the following PLP - catalyzed \beta, \gamma eliminationarrow_forwardThe nitrogenase complex can reduce molecules other than N2. Provide the structures for the products for each of the following substrates (real and hypothetical) that contain triple bonds: hydrogen cyanide, dinitrogen, and acetylene.arrow_forwardQuestion #1: Choanoflagelletes are a unicellular ancestor to animals. One observation to support this hypothesis is the appearance of adhesion molecules that are key to the development of multiceullarity. Bulk transport Gap junctions Animals. Adhesion, cell signaling Single-celled :} Insects, mammals, and other animals with bilateral symmetry (~10,000,000) Jellyfish and their relatives (10,000) } Sponges (10,000) Choanoflagellates (150) Despite their simple unicellular lifestyle they express adhesion molecules including cadherins and lectins (King et al., 2003) but don't seem to have molecules that are typically found in the extracellular matrix such as integrins or laminins (Williams et al., 2014). Design a microscopy experiment to test the assertion that choanoflagellates have (some) adhesion molecules and those molecules play a similar role in a closely related animal like sponges.arrow_forward
- Lactate dehydrogenase is a tetramer of MW 134000 g/mole composed of subunits which are equal in size. It is found in the cytoplasm of eukaryotic and prokaryotic cells. Describe the secondary, tertiary and quaternary structures of lactate dehydrogenase.arrow_forwardDecoupling agents such as 2,4-DNP can result in altered metabolic activity. Explain what 2,4-DNP is, describe how it alters metabolic activity, and why this could be dangerous.arrow_forwardPlz answer correctly do not copy. Question- Arsinate binds to reduced thio groups such as those found in cystiene residues in proteins, lipoate or glutathione. The resulting inhibition leads to central nervous system pathologies. The binding of arsinate to the dihydrolipoyl groups inhibits which of the following enzyme(s)? succinate dehydrogenase malate dehydrogenase pyruvate dehydrogenase complex branched-chain amino acid dehydrogenase complex isocitrate dehydrogenase alpha-ketoglutarate dehydrogenase complexarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning