Concept explainers
To review:
The tracking of intracellular molecules by super-resolution imaging.
Introduction:
Super-resolution imaging is the process by which intracellular molecules can be tracked live inside a living system especially a bacterial cell. The process of transport of macromolecules during intracellular events can be easily tracked with the help of specific dyes that can bind to target molecules. The properties of these dyes to absorb and emit light at different wavelengths in visible light can help track many intracellular events.
Super-resolution imaging combines the properties of fluorescent color absorbing dyes and emitting dyes to track live intracellular events like cell division, deoxyribose
Another application of this method is the use of green and yellow fluorescent dye to track two key proteins par A and par C involved in bacterial transition between stalked and flagella cells. The movement of the fluorescence across the cell can be tracked because of the different colored dyes confirming the role of the two proteins in the process by live tracking. The emission of light is also not uniform in case fluorophore emits a light.
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Microbiology: An Evolving Science (Fourth Edition)
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