Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 2.3, Problem 2TQ
Summary Introduction
To review:
The parameters determining that the cell under microscopic observation is hollow or is simply out of focus.
Introduction:
The observation of a bacterial specimen depends upon the relative distance of the object with the focal point. The resolution of the specimen or the object also changes because of an alteration of the focal point. Maximum resolution of the specimen can be obtained from the focal point.
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In the micrograph below, you are seeing a collection of cells that have been stained with fluorescent dyes. One of the dyes appears green, and the other dye appears blue (when viewed with a fluorescence microscope).
Based on the appearance of these stained cells, which of the following cell structures must the GREEN dye be staining?
Endoplasmic reticulum
Cytoskeleton
Flagella
Mitochondria
Nucleus
..
Which electron or light microscopy technique would you use for the following? (also explain)
For visualizing the fine structure of cellular organelles and cell architecture in the cell interior using very thin cross-sections made in Epon.
To obtain sequential images of a macromolecule in order to study its activity in real-time.
To obtain an extremely clear three-dimensional image of the nucleus of a single cell.
What is the magnification of the image in a microscope using a 20x ocular and a 100x objective?
Let’s say you wanted to look at cells of Bacillus cereus, which are rod-shaped cells that are about 4 µm long. If you were observing B. cereus with a microscope using the high-power lens, how big would the cells appear to be when you look at them?
Chapter 2 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 2.1 - Prob. 1TQCh. 2.1 - Prob. 2TQCh. 2.2 - Prob. 1TQCh. 2.3 - Prob. 1TQCh. 2.3 - Prob. 2TQCh. 2.4 - Prob. 1TQCh. 2.5 - Prob. 1TQCh. 2.5 - Prob. 2TQCh. 2.6 - Prob. 1TQCh. 2.6 - Prob. 2TQ
Ch. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - Prob. 4RQCh. 2 - Prob. 5RQCh. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Prob. 9RQCh. 2 - Prob. 10RQCh. 2 - Prob. 11RQCh. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - Prob. 1TQCh. 2 - Prob. 2TQCh. 2 - Prob. 3TQCh. 2 - Prob. 4TQ
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- You are looking at a larger cell (approximately 50 micrometers) under a microscope, and see a nucleus and membrane-bound organelles. What type of cell can you confirm you are observing?arrow_forwardIn the micrograph below, you are seeing a collection of cells that have been stained with fluorescent dyes. One of the dyes appears green, and the other dye appears blue (when viewed with a fluorescence microscope). Examine it and answer the question below. Based on the appearance of these stained cells, which of the following cell structures must the GREEN dye be staining? Endoplasmic reticulum Cytoskeleton Flagella Mitochondria Nucleus .arrow_forward(a) If you were to magnify a cell 10,000-fold (typical of the magnification achievedusing an electron microscope), how big would it appear? Assume you are viewing a“typical” eukaryotic cell with a cellular diameter of 50 μm.(b) If this cell were a muscle cell (myocyte), how many molecules of actin could ithold? Assume the cell is spherical and no other cellular components are present; actinmolecules are spherical, with a diameter of 3.6 nm. (c) If this were a liver cell (hepatocyte) of the same dimensions, how manymitochondria could it hold? Assume the cell is spherical; no other cellular components arepresent; and the mitochondria are spherical, with a diameter of 1.5 μmarrow_forward
- You are examining a cell from a crime scene using an electron microscope. It contains ribosomes, DNA, plasma membrane, cell wall, and mitochondria. What type of cell is it?arrow_forwardYou are studying amoebas and paramecia, two different single-celled protozoans. You have at your disposal a standard white light (known as "bright-field") microscope, a fluorescence microscope, a scanning electron microscope, and a transmission electron microscope. For each of the following studies, indicate which type of microscope would be most useful. Items (6 items) (Drag and drop into the appropriate area below) Take movies of unmanipulated cells swimming or crawling in a drop of water. Categories View three- dimensional images of the outer surface of a paramecium, including cilia. bright-field Drag and drop here View cytoplasmic streaming (movement of organelles within the cytoplasm) in a paramecium. fluorescence Drag and drop here Stain and visualize chromosomes and mitotic spindles in dividing amoebae. Visualize ribosomes in the cytosol and mitochondria of amoebas. scanning electron Drag and drop here Use labeled antibodies to locate and make color images of lysosomes in both…arrow_forwardThe Size of Cells and Their Components: a. (a) If you were to magnify a cell 10,000 fold (typical of the magnification achieved using an electron microscope), how big would it appear? Assume you are viewing a "typical" eukaryotic cell with a cellular diameter of 50 um.arrow_forward
- If you could not use a microscope, how would you determine whether a cell is prokaryotic or eukaryotic ? Assume the organism can be cultured easily in the laboratory.arrow_forwardWhat is the visible structure at the edge of the type of cell represented by "B"? A. В. С. O cell wall O cytoplasm nucleus O plasma membranearrow_forwardA) The size of a cell seen under a microscope is 3mm Using a total magnification of 40x. What is the actual cell size and decide if the cell is prokaryote or eukaryote based on its size?arrow_forward
- Which of the following is a function performed by the cytoskeleton?a) provides structural support that determines cell shape and resists deforming forcesb) positions various organelles within the cell interiorc) provides a network of tracks over which materials like mRNA and organelles move within cellsd) serves as a force-generating apparatus that moves cells from one place to anothere) all of these choicesarrow_forwardThe cell membrane a) has no function that is known. b) is responsible for starting mitosis. c) controls what substances enter the cell. d) has a thick cuticle around the outside.arrow_forwardFor the following questions referring to cell types and structures, please use the image below for reference. Questions that refer to Cell #1, Cell #2, or Cell #3 are indicating that you should look at the following image to help answer the question. Cell #1 Cell #2 Cell #3 Cell membrane Nucleus - Nucleus DNA Cell Wall Cell Wall Mitochondrion : Cll Mitochondrion membrane Cll Chloroplast membranearrow_forward
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