Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 2.3, Problem 1TQ
Summary Introduction
To review:
The angle θ that might result in magnification greater than 100X from a single lens and the practical problems that might come in designing such a lens.
Introduction:
Single lens microscopes rely on a single bending of refracted light and one-time magnification. An increase in the angle of aperture will increase the intensity of the light cone produced, will enhance the size of the image and ultimately the resolution. However, there are limits to which this angle of aperture could be increased.
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Chapter 2 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 2.1 - Prob. 1TQCh. 2.1 - Prob. 2TQCh. 2.2 - Prob. 1TQCh. 2.3 - Prob. 1TQCh. 2.3 - Prob. 2TQCh. 2.4 - Prob. 1TQCh. 2.5 - Prob. 1TQCh. 2.5 - Prob. 2TQCh. 2.6 - Prob. 1TQCh. 2.6 - Prob. 2TQ
Ch. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - Prob. 4RQCh. 2 - Prob. 5RQCh. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Prob. 9RQCh. 2 - Prob. 10RQCh. 2 - Prob. 11RQCh. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - Prob. 1TQCh. 2 - Prob. 2TQCh. 2 - Prob. 3TQCh. 2 - Prob. 4TQ
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- The pupil of a person's eye changes from a diameter of 3.5 mm to 1.5 mm as the illumination is increased. By what factor does the minimum angle of resolution change?arrow_forwardSuppose you measure the scanning (4x) field of vision diameter with a stage micrometer and it is 2 mm. If high power is 40X, and gives 10X more magnification than scanning power, how big will the diameter of the field of vision be using high power?arrow_forwardWhat is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.arrow_forward
- To obtain a 400X magnification image you may choose a 40X objective lens with a 10X projector lens, or a 20X objective lens with a 20X projector lens. What are the differences in their image quality?arrow_forwardAnswer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's Lawarrow_forwardThe least distance of distinct vision for an eye lens is caused by the action of the:(a) 25 m(b) 2.5 cm(c) 25 cm(d) 2.5 marrow_forward
- Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?arrow_forwardIn a converging lens, if an incident ray is parallel to the principle axis, the reflected ray ____________. 2. In a diverging lens, if an incident ray is parallel to the principle axis, the reflected ray ____________. 3. Can a real image be obtained by means of a diverging lens? If yes, how?arrow_forward4. 45 year old man can see with his left eye between 25 →300 cm and in his right eye between 15cm→ ∞. a man has astigmatism at his left eye which requires a lens of focal length +0.5m at axis 120°. The patient requires magnification glasses of focal length 0.25m. write the required prescription for this patientarrow_forward
- How can you improve contrast when viewing a specimen?. What should you do if you have difficulty focusing in high power?. Why is important to use the fine adjustment nob only when focusing while using the high dry objective lens?arrow_forwardwhich objective lens is in place if the object you are viewing is magnified 1000x? (assume ocular lens magnification = 10x)arrow_forwardThe field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?arrow_forward
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