Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Question
Chapter 2, Problem 3TQ
Summary Introduction
To review:
The appearance of artifacts in microscopic images and the methodology to classify optical artifacts and an actual feature of an image.
Introduction:
The focusing of an object is the process by which the settings of the eyepiece are adjusted in order to get a clear image. The ability to concentrate the light rays and view a clear image depends upon how close or far the specimen is placed from the central point where the light rays converge. Any sort of interference while preparing the sample especially in high-resolution microscopes can affect the outcome.
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Chapter 2 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 2.1 - Prob. 1TQCh. 2.1 - Prob. 2TQCh. 2.2 - Prob. 1TQCh. 2.3 - Prob. 1TQCh. 2.3 - Prob. 2TQCh. 2.4 - Prob. 1TQCh. 2.5 - Prob. 1TQCh. 2.5 - Prob. 2TQCh. 2.6 - Prob. 1TQCh. 2.6 - Prob. 2TQ
Ch. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - Prob. 4RQCh. 2 - Prob. 5RQCh. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Prob. 9RQCh. 2 - Prob. 10RQCh. 2 - Prob. 11RQCh. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - Prob. 1TQCh. 2 - Prob. 2TQCh. 2 - Prob. 3TQCh. 2 - Prob. 4TQ
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- Which of the following is true about the relationship between depth of field and magnification? There is no relationship. When magnification increases, depth of field decreases. When magnification increases, depth of field increases. Stereomicroscopes offer a lesser depth of field than compound microscopes. The depth of field of a microscope determines how much magnification it can achieve.arrow_forwardDefine each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?arrow_forwardCalculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4,500 um Scanning (40X): 1.8 mm x 100X/40X = 5 mm = %3D Low power (100X): FOV diameter = 1.8 mm = 1,800 um 1.8 mm x 100X/400X = 0.45 mm =450 um 180 um High power (400X): 1.8 mm x 100X/1000x = 28 mm = Oil immersion (1000X): %3D Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification FOV diam. um FOV diam. um length um length umarrow_forward
- Explain why an image must be centered in the field of view when using low power before moving to a high power magnification.arrow_forwardIn light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?arrow_forwardYou should always position the cells or tissues in the center of view before increasing magnification, because... You know that the image will move right as you move the slide to the left. You know that the image will move up as you move the slide down. You know that the center of the field of view will be unchanged. This is the best way to avoid breaking the slide with an objectivearrow_forward
- What is the diffraction-limited optical resolution of the human eye? (feel free to find the geometrical and optical properties of the eye using literature search)arrow_forwardDifferentiate between the concepts of magnification, refraction, andresolution and explain how they contribute to the clarity of an image.arrow_forwardSuppose you measure the scanning (4x) field of vision diameter with a stage micrometer and it is 2 mm. If high power is 40X, and gives 10X more magnification than scanning power, how big will the diameter of the field of vision be using high power?arrow_forward
- PRELAB: Calculating Magnification Each lens makes the object look larger by a specified amount. To determine the total magnification, multiply the magnification of the eyepiece and the objective being used. Table 1: Microscope Magnification Magnifying power Objective Objective Eyepiece Total name Scanner 4 X Low power 100 X High power 40 χ PRELAB: Estimating the Size of an Object using a Microscope You will not be able to use a ruler to directly measure the size of a cell. Instead, you will use an indirect method by comparing the cell to the size of something you already know. The diameter of the microscope's field of view as seen through the eyepiece is a convenient length to use. A very small unit of measurement, the micron (µm) is used for measuring microscopic objects. The conversions are: 1 um = 0.001 mm 1 mm = 1000 µm Table 2: Field of View Diameters Objective Diameter name millimeter (mm) micron (um) Scanner 4 Low power High power 0.5 To estimate the size of an object, divide…arrow_forwardAnswer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's Lawarrow_forwardTo obtain a 400X magnification image you may choose a 40X objective lens with a 10X projector lens, or a 20X objective lens with a 20X projector lens. What are the differences in their image quality?arrow_forward
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