Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Question
Chapter 2, Problem 2TQ
Summary Introduction
To review:
The increased resolution due to increased magnification and the inability of unaided eyes to resolve microscopic objects. Also, mention the reason behind the limit of magnification.
Introduction:
Observing an object with unaided eyes leads to a natural process of image enhancement due to bending of the incident light on a solid surface like the eye lens. This takes place due to many factors like the density of the medium, the curvature of the lens, and the angle of aperture. Enhancing the size of an image affects the sharpness and clarity with which the object can be viewed.
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Chapter 2 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 2.1 - Prob. 1TQCh. 2.1 - Prob. 2TQCh. 2.2 - Prob. 1TQCh. 2.3 - Prob. 1TQCh. 2.3 - Prob. 2TQCh. 2.4 - Prob. 1TQCh. 2.5 - Prob. 1TQCh. 2.5 - Prob. 2TQCh. 2.6 - Prob. 1TQCh. 2.6 - Prob. 2TQ
Ch. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - Prob. 4RQCh. 2 - Prob. 5RQCh. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Prob. 9RQCh. 2 - Prob. 10RQCh. 2 - Prob. 11RQCh. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - Prob. 1TQCh. 2 - Prob. 2TQCh. 2 - Prob. 3TQCh. 2 - Prob. 4TQ
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- The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?arrow_forwardTo obtain a 400X magnification image you may choose a 40X objective lens with a 10X projector lens, or a 20X objective lens with a 20X projector lens. What are the differences in their image quality?arrow_forwardWhich light source will provide you with greater resolution than green light, blue or red light? why?arrow_forward
- Explain the difference between resolution and magnification.arrow_forwardDefine the terms magnification and resolution.arrow_forwardWhy are stereomicroscopes often referred to as dissecting microscopes? Stereomicroscopes come apart (dissect) easily. The large distance between the lens and the specimen makes them ideal for dissections. They are good for viewing much smaller objects. Only a few specimens will fit under the stereomicroscope. The two eyepiece lenses on a stereomicroscope give a better perception of depth.arrow_forward
- Use the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?arrow_forwardWhat did you notice about the Scalp Bald Human photomicrographs shown at (a) 40x vs (b) 100x TM? Why do you think your instructor is asking you to look at it at two different total magnifications?arrow_forwardwhich objective lens is in place if the object you are viewing is magnified 1000x? (assume ocular lens magnification = 10x)arrow_forward
- While looking into your compound light microscope you find that only part of the specimen is visible. What might be the cause? Question options: Insufficient illumination from the substage condenser. Iris diaphragm has not been properly adjusted. Your objective lens has not clicked fully into position. Your objective lens is dirty.arrow_forwardIn light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?arrow_forward
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