Concepts of Genetics (12th Edition)
Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 19, Problem 22ESP
Summary Introduction

To determine: The histone H3 modifications that are associated with the activation of the gene.

Introduction: Histone modifications take place at specific amino acids in the N-terminal tail of histones H3, H4, 2A, and 2B.

Summary Introduction

To determine: The H3 modifications that are associated with repression of gene expression.

Introduction: The sum of the complex patterns and interactions within the histone molecules is known as the histone code.

(a)

Summary Introduction

To determine: Whether there are any overlaps on the lists of H3 modifications.

Introduction: Over twenty different chemical modifications can be made to histones. The major alterations include the addition of acetyl, phosphate, and methyl groups.

(b)

Summary Introduction

To determine: Whether the overlaps can be explained by different modifications.

Introduction: Histone modifications are covalent posttranscriptional modifications of amino acids near the N-terminal ends of histone proteins.

(c)

Summary Introduction

To determine: The other way to explain the overlaps.

Introduction: Amino acids are considered as building blocks of protein. Lysine is an amino acid. The process of formation of proteins from RNA is known as translation.

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Explain how the following mutations would affect transcription of the yeast GAL1 gene in the presence of galactose. (a) A deletion within the GAL4 gene that removes the region encoding amino acids 1 to 100. (b) A deletion of the entire GAL3 gene. (c) A mutation within the GAL80 gene that blocks the ability of Gal80 protein to interact with Gal3p. (d) A deletion of one of the four UASG elements upstream from the GAL1 gene. (e) A point mutation in the GAL1 core promoter that alters the sequence of the TATA box.
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