Concepts of Genetics (12th Edition)
Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 19, Problem 1PDQ

(a)

Summary Introduction

To determine: The way in which methylation of promoters silences gene expression.

Introduction: Methylation can be defined as the process of adding on a methyl group to a molecule of DNA. The process of methylation results in the modification of DNA activity, but it does not change the DNA sequence.

(b)

Summary Introduction

To determine: The evidence that epigenetic changes are involved in cancer.

Introduction: Epigenetics refers to the study of alteration in the expression of genes. The changes in gene expression do not alter the DNA sequence that is only the phenotype is altered without alteration in the genotype.

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We know that eukaryote gene regulation can occur at any point in the process of gene expression.   (a) What is the final step where gene expression control may occur in the process of protein expression ?   (b) Is this a cellular energy efficient or inefficient mechanism of gene regulation? Explain.
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You are interested in studying a novel gene that appears to be involved in cancer. There is no information about the function of this gene. What would you do to obtain the cDNA for this gene? How would you express this gene and what expression systems might you utilize to study its function and why? How would determine the subcellular localization of this gene in eukaryotic cells? What are alternative methods in case one doesn't work? How would you purify and determine the 3-dimensional structure of this protein?
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QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY