Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 16, Problem 6PDQ
For the genotypes and conditions (lactose present or absent) shown in the following table, predict whether functional enzymes, nonfunctional enzymes, or no enzymes are made.
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Draw the α-l-glucopyranose sugars using Haworth projections:
The enzyme glucose oxidase isolated from the mold Penicillium notatum catalyzes the oxidation of β-Dglucose to D-glucono-δ-lactone. This enzyme is highly specific for the β anomer of glucose and does not affect the α anomer. In spite of this specificity, the reaction catalyzed byglucose oxidase is commonly used in a clinical assay for total blood glucose—that is, for solutions consisting of a mixture of β- and α-D-glucose. What are the circumstances required to make this possible? Aside from allowing the detection of smaller quantities of glucose, what advantage does glucose oxidase offer over Fehling’s reagent for measuring blood glucose?
The enzyme glucose oxidase isolated from the mold Penicillium notatum catalyzes the oxidation of
B-D-glucose to D-glucono-6-lactone. The enzyme is highly specific for the B anomer of glucose and does
not affect the a anomer. In spite of this specificity, the reaction catalyzed by glucose oxidase is
commonly used in a clinical assay for total blood glucose -that is, for solutions consisting of a mixture of
B- and a-D-glucose, as well as other sugars present in blood. The oxidation proceeds in the presence of
oxygen and forms hydrogen peroxide, in addition to the lactone. A second enzyme, called peroxidase,
catalyzes the reaction of hydrogen peroxide with colorless compounds to create a colored product,
which is quantified with a simple spectrophotometer. What are the circumstances required to make this
possible? Aside from allowing the detection of smaller quantities of glucose, what advantage does
glucose oxidase offer over the Fehling's reagent for measuring blood glucose?
Chapter 16 Solutions
Concepts of Genetics (12th Edition)
Ch. 16 - Even though the lac Z, Y, and A structural genes...Ch. 16 - Predict the level of genetic activity of the lac...Ch. 16 - Prob. 1CSCh. 16 - Prob. 2CSCh. 16 - Prob. 3CSCh. 16 - HOW DO WE KNOW? In this chapter, we focused on the...Ch. 16 - Prob. 2PDQCh. 16 - Contrast positive versus negative control of gene...Ch. 16 - Contrast the role of the repressor in an inducible...Ch. 16 - For the lac genotypes shown in the following...
Ch. 16 - For the genotypes and conditions (lactose present...Ch. 16 - The locations of numerous lacI and lacIS mutations...Ch. 16 - Prob. 8PDQCh. 16 - Prob. 9PDQCh. 16 - Predict the effect on the inducibility of the lac...Ch. 16 - Erythritol, a natural sugar abundant in fruits and...Ch. 16 - Prob. 12PDQCh. 16 - Prob. 13PDQCh. 16 - Neelaredoxin is a 15-kDa protein that is a gene...Ch. 16 - The creation of milk products such as cheeses and...Ch. 16 - Both attenuation of the trp operon in E. coli and...Ch. 16 - Prob. 17PDQCh. 16 - Prob. 18ESPCh. 16 - In a theoretical operon, genes A, B, C, and D...Ch. 16 - A bacterial operon is responsible for the...Ch. 16 - A marine bacterium is isolated and shown to...Ch. 16 - Prob. 22ESPCh. 16 - Prob. 23ESP
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