Concepts of Genetics (12th Edition)
Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 16, Problem 22ESP

(a)

Summary Introduction

To describe: The two different mutations which would result in auvrA constitutive phenotype and mention the actual genotypes involved.

Introduction: Mutation is the process of changes at the genetic level in which the coding of the DNA is altered by the chemical, radiation, and environmental factors. These factors are termed as mutagens. Mutagens are capable to induce mutation in an individual.

(b)

Summary Introduction

To describe: The outlined series of a genetic experiment that makes use of diploid strains for the determination of the possible mutations that have been isolated.

Introduction: Mutations are the changes in the sequence of DNA which may be harmful or useful for an individual. It leads to evolution and the better survival of an individual. The mutations can be inherited or acquired.

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The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism.  Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products.  These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type   Detail the functionality of the lac operon under the following genetic and cellular conditions.  You do not need to consider the role of CAP in your analysis.      Genes Repressed (yes/no) Constitutive (yes/no) IS  O+  Z+       IS  OC  Z+
E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a single promoter and transcribed as one long gene. The presence of tryptophan shut down the production of tryptophan by the cell by binding to the repressor. This changes the repressors shape allowing it to bind to DNA operator, blocking RNA polymerase and cutting off the production of tryptophan. a. Describe what would happen to the operon if some of the cells had a mutation on the repressor, not allowing it to bind with tryptophan. The repressor is described as an allosteric protein. What does this mean? b. Does the tryptophan model demonstrate an inducible or repressible operon? What is your evidence?*
A researcher discovered Suramin as a potent and selective inhibitor of Mycobacterium tuberculosis RecA protein and the SOS response. What would be the outcome if the action of RecA was inhibited during the SOS response? O LexA would not autolyse, and therefore the transcription of DNA repair genes would not occur; the bacteria would likely not survive. O LexA would autolyse, and therefore the transcription of repair genes would not occur; that would be lethal to the bacteria. LexA would autolyse, and therefore the transcription of DNA repair genes would occur; and the bacteria will be susceptible to antibiotics. LexA would not autolyse, and therefore the transcription of repair genes would occur; essentially that would be lethal to the bacteria.
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QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY