Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 16, Problem 32P

In addition to the pathway described in Figure 16.31, eukaryotes can also synthesize phosphatidylcholine and phosphatidylethanolamine, starting with free choline or ethanolamine, respectively. In this salvage pathway, the phosphodiester linkage in the glycerophospholipid is formed by activating the -OH of the head group instead of the diacylglycerol moiety. Propose a pathway for the synthesis of phosphatidylcholine from free choline using this salvage route.

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An enzyme that catalyzes disulfide– sulfhydryl exchange reactions, called protein disulfide isomerase (PDI), has been isolated. PDI rapidly converts inactive scrambled ribonuclease into enzymatically active ribonuclease. In contrast, insulin is rapidly inactivated by PDI. What does this important observation imply about the relation between the amino acid sequence of insulin and its threedimensional structure?
Write schematically the synthesis of phosphatidylcholine from phosphatidylethanolamine. What compounds are necessary for this biochemical process? Biological significance of phospholipids.
Glucose-1-phosphate has a higher phosphoryl group transferpotential than does glucose-6-phosphate. Review the structures of these molecules and suggest a reason for thisphenomenon.
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