Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 16, Problem 19TYK
You are trying to test your hypothesis that
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When DNA replication was investigated by using heavy, N15 DNA to mark the original molecules, and light, N14 DNA to mark the newly synthesized molecules, one band was found in the middle of the centrifuge column after one round of replication, and two bands were found (middle and top of column) after 2 rounds of replication. Imagine that after 1 round of replication 2 bands were found, one at the bottom and one at the top of the centrifuge column. In that case, what model of DNA replication would have been supported?
The dispersive model
The conservative model
The Franklin model
The semi-conservative model
for the lagging strand, is it the upper or the lower?
You decide to repeat the Meselson-Stahl experiment, except this time you plan to grow the E. coli cells on light 14N medium for many generations and then transfer them to heavy 15N medium and allow them to grow for 2 additional generations (2 rounds of DNA replication). If the conservative model of DNA replication was correct, what is the expected distribution of DNA in the density gradient after two rounds of replication?
Multiple Choice
One band of intermediate density.
One band of light density.
One band of heavy density.
One band of light density and one band of heavy density.
One band of light density and one band of intermediate density.
Chapter 16 Solutions
Study Guide for Campbell Biology
Ch. 16 - Hershey and Chase devised an experiment using...Ch. 16 - Review the structure of DNA by labeling the...Ch. 16 - Using different colors for heavy (parental) and...Ch. 16 - Look back to Interactive Question 16.2 and label...Ch. 16 - In this diagram of bacterial DNA replication,...Ch. 16 - Draw the last Okazaki fragment being formed on the...Ch. 16 - List the successive levels of packing in a...Ch. 16 - Prob. 1SYKCh. 16 - Prob. 2SYKCh. 16 - One of the reasons most scientists thought...
Ch. 16 - Transformation involves a. the uptake of external...Ch. 16 - Prob. 3TYKCh. 16 - Which of the following most closely represents...Ch. 16 - Prob. 5TYKCh. 16 - Prob. 6TYKCh. 16 - In their classic experiment, Meselson and Stahl a....Ch. 16 - The joining of nucleotides in the polymerization...Ch. 16 - DNA polymerase is not able to begin copying a DNA...Ch. 16 - Prob. 10TYKCh. 16 - Prob. 11TYKCh. 16 - Prob. 12TYKCh. 16 - Which of the following is least related to the...Ch. 16 - Prob. 14TYKCh. 16 - Prob. 15TYKCh. 16 - Prob. 16TYKCh. 16 - Prob. 17TYKCh. 16 - Which of the following statements about telomeres...Ch. 16 - You are trying to test your hypothesis that DNA...Ch. 16 - Given the experimental procedure explained in...Ch. 16 - Prob. 21TYKCh. 16 - Biologists have learned from the technique of...
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- You decide to repeat the Meselson-Stahl experiment, except this time you plan to grow the E. coli cells on light 14N medium for many generations and then transfer them to heavy 15N medium and allow them to grow for 2 additional generations (2 rounds of DNA replication). If the conservative model of DNA replication was correct, what is the expected distribution of DNA in the density gradient after two rounds of replication?arrow_forwardLook at the double-stranded segment of DNA shown below. Imagine that the two strands have already been denatured, and the temperature has been decreased to an appropriate annealing temperature. Show where the two primers would anneal to the strands, then indicate the direction of extension on each new strand with an arrow. 5’--T C A G G A C G T A A G C T T G C A T A T C T C G A T G C T A A A T C A T—3’ 3’--A G T C C T G C A T T C G A A C G T A T A G A G C T A C G A T T T A G T A—5’ Primer #1: 3’ A C G A T T T 5’ Primer #2: 5’ G G A C G T A 3’arrow_forwardConsider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.arrow_forward
- A scientist successfully analyzed a new micro-organism. Because this micro-organism contains double-stranded DNA as genetic material, Meselson-Stahl techniques was employed. The following shows the results of the experiment where L – light chain (14N) and H – heavy chain (15N).What is the mechanism of replication in this organism in the picture? Explain how you got the answer. The following piece of DNA is sequenced using the dideoxy method: 3’-AAGCGGCTAATCC-5’. Accidentally, you forget to include dATP in the four reactions that contain a ddNTP. What is the sequence of the daughter strand produced from this sequencing activity? Show the process. The following piece of DNA is sequenced using the dideoxy method: 3’-AAGCGGCTAATCC-5’. Accidentally, you forget to include dATP in the four reactions that contain a ddNTP. How many bands will appear in the lane containing ddATP? Show the process. The following piece of DNA is sequenced using the dideoxy method: 3’-AAGCGGCTAATCC-5’.…arrow_forwardUse the picture to solve for the following.arrow_forwardOne strand of a single DNA helix is labeled red while the other strand of the same DNA helix is labeled blue. This double helix DNA is replicated through the process of semi-conservative replication. Note that a completely newly synthesized strand of DNA will be white. After three rounds of DNA replication, how many all-white DNA (both strands are white) will there be? Explain your reasoning.arrow_forward
- In an experiment, you grow bacterial cells for several generations in the presence of a light isotope of carbon (C¹2) and then transfer them into a medium containing a heavy isotope of carbon (C¹³). After one round of DNA replication, which of the following statements is true? O All DNA molecules will be labeled entirely with C¹3 ● For all DNA molecules, each strand will be labeled with a mixture of C¹2 and C¹³. Half the DNA molecules will be labeled with C¹2 only, whereas the other half will be labeled with C¹3 only. O All DNA molecules will be labeled entirely with C¹2 O For all DNA molecules, one strand will be entirely labeled with C¹2, whereas the other strand will be entirely labeled with C¹3 O Insufficient information to conclude.arrow_forwardConsider the following segment of DNA, which is part of a linear chromosome: LEFT 5’.…TGACTGACAGTC….3’ 3’.…ACTGACTGTCAG….5’ RIGHT During DNA replication, this double-strand molecule is separated from the right to the left into two single strands and the replisome is moving from the right to the left of the segment. The replisome is approaching to a chromosomal end on the left. Considering this left chromosomal end, if without telomerase, the newly synthesized daughter DNA of ___________ will be shortened? neither top or bottom strand both top and bottom strands the top strand the bottom strandarrow_forwardDNA replication occurs by adding (Note: NTPS = nucleotide triphosphates; dNTPs = deoxynucleotide triphosphates) DNTPS to the 3' end of the template strand NTPS to the 3' end of the daughter strand DNTPS to the 3' end of the daughter strand DNTPS to the 5' end of the template strand NTPS to the 5' end of the daughter strandarrow_forward
- What are the three models of DNA replication? With the aid of illustrations, show how the Meselson Stahl experiment come to the conclusion of one model of DNA replication. Is DNA replication bidirectional? How did you arrive at this conclusion? Explain the bacterial replication model that supports this conclusion.arrow_forwardIn the diagram below, the arrow indicates the direction of movement of a replication fork. O Top; Top (a) Which template strand (top or bottom) is the lead strand? (b) Which strand (top or bottom) would you expect to find the Okazaki fragments? O Bottom; Top Replication fork Bottom; Bottom O Top; Bottom NK Unwinding. ?arrow_forward3' 5' Shown is a segment of DNA about to be replicated. a) What is the sequence of the complementary parent strand? > Be sure to label the 3' and 5' to the ends of your strand. Determine the leading strand. b) What is the sequence of the copied leading strand? > Include an RNA primer at the beginning of the strand that is 4 nucleotides in length. (Box/Highlight/Bold this segment) Be sure to label the 3' and 5' to the ends of your strand. 5' 3' Direction of Replicationarrow_forward
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